Effects of selective nitric oxide synthase inhibition in hyperdynamic endotoxemia in dogs

Antal Wolfárd, J. Kaszaki, Csaba Szabo, Z. Balogh, S. Nagy, M. Boros

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives: Our aims were to investigate the systemic hemodynamic effects of constitutive endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) inhibitors in hyperdynamic endotoxemia. Patients and Methods: Group 1 comprised sham-operated controls, while in group 2, 3 and 4, a hyperdynamic circulatory reaction was elicited by a 2-hour infusion of Escherichia coli endotoxin (ETX) in a dose of 5.3 μg/kg. The animals in group 3 were treated with 12.5 mg/kg nonselective NOS inhibitor N-w-nitro-L-arginine methyl ester (L-NAME), and those in group 4 with 2 mg/kg of the specific iNOS inhibitor S-methyl-isothiourea (SMT). Mean arterial pressure (MAP), cardiac output (CO) and myocardial contractility (MC) were measured, and total peripheral resistance (TPR) was calculated. The eNOS and iNOS activities were determined in myocardial biopsy samples taken after 8 h of endotoxemia. Results: ETX induced significant decreases in TPR and MAP, a transient myocardial depression, and increased the myocardial eNOS and iNOS activities. L-NAME decreased the activities of both isoenzymes, increased MC but induced a fall in CO. SMT inhibited iNOS by 60%, without influencing the eNOS activity, increased MAP and contractility in the early phase of endotoxemia, and induced only a slight decrease in CO. Conclusions: Nonselective NOS inhibition restores the arterial pressure and exerts a positive inotropic effect, but decreases CO. SMT selectively decreases the iNOS activation without disturbing the vasoregulatory function of the eNOS-derived nitric oxide in hyperdynamic endotoxemia in the dog.

Original languageEnglish (US)
Pages (from-to)314-323
Number of pages10
JournalEuropean Surgical Research
Volume31
Issue number4
DOIs
StatePublished - Jul 1999
Externally publishedYes

Fingerprint

Endotoxemia
Nitric Oxide Synthase Type III
Nitric Oxide Synthase
Cardiac Output
Dogs
Arterial Pressure
NG-Nitroarginine Methyl Ester
Vascular Resistance
Endotoxins
Isoenzymes
Nitric Oxide
Hemodynamics
Biopsy
S-methylisothiopseudouronium

Keywords

  • Heart
  • Myocardial contractility
  • N-ω-nitro-L-arginine methyl ester
  • Nitric oxide synthase activity
  • S-methylisothiourea

ASJC Scopus subject areas

  • Surgery

Cite this

Effects of selective nitric oxide synthase inhibition in hyperdynamic endotoxemia in dogs. / Wolfárd, Antal; Kaszaki, J.; Szabo, Csaba; Balogh, Z.; Nagy, S.; Boros, M.

In: European Surgical Research, Vol. 31, No. 4, 07.1999, p. 314-323.

Research output: Contribution to journalArticle

Wolfárd, Antal ; Kaszaki, J. ; Szabo, Csaba ; Balogh, Z. ; Nagy, S. ; Boros, M. / Effects of selective nitric oxide synthase inhibition in hyperdynamic endotoxemia in dogs. In: European Surgical Research. 1999 ; Vol. 31, No. 4. pp. 314-323.
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abstract = "Objectives: Our aims were to investigate the systemic hemodynamic effects of constitutive endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) inhibitors in hyperdynamic endotoxemia. Patients and Methods: Group 1 comprised sham-operated controls, while in group 2, 3 and 4, a hyperdynamic circulatory reaction was elicited by a 2-hour infusion of Escherichia coli endotoxin (ETX) in a dose of 5.3 μg/kg. The animals in group 3 were treated with 12.5 mg/kg nonselective NOS inhibitor N-w-nitro-L-arginine methyl ester (L-NAME), and those in group 4 with 2 mg/kg of the specific iNOS inhibitor S-methyl-isothiourea (SMT). Mean arterial pressure (MAP), cardiac output (CO) and myocardial contractility (MC) were measured, and total peripheral resistance (TPR) was calculated. The eNOS and iNOS activities were determined in myocardial biopsy samples taken after 8 h of endotoxemia. Results: ETX induced significant decreases in TPR and MAP, a transient myocardial depression, and increased the myocardial eNOS and iNOS activities. L-NAME decreased the activities of both isoenzymes, increased MC but induced a fall in CO. SMT inhibited iNOS by 60{\%}, without influencing the eNOS activity, increased MAP and contractility in the early phase of endotoxemia, and induced only a slight decrease in CO. Conclusions: Nonselective NOS inhibition restores the arterial pressure and exerts a positive inotropic effect, but decreases CO. SMT selectively decreases the iNOS activation without disturbing the vasoregulatory function of the eNOS-derived nitric oxide in hyperdynamic endotoxemia in the dog.",
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