Effects of serum from Neurocysticercosis patients on the structure and viability of Taenia solium oncospheres

J. L. Molinari, P. Tato, R. Lara-Aguilera, A. Clinton White

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Neurocysticercosis, caused by Taenia solium is arguably the most common parasitic disease of the central nervous system. In taeniid infections of nonhuman mammals, there is strong evidence of immunity in the intermediate host to the invasive larvae (oncospheres). This immunity, which is mediated by antibody and complement, has been exploited to develop vaccines that effectively prevent infection. To examine the immune response in humans, T. solium eggs were hatched and activated in vitro. Activated oncospheres were incubated with heat-inactivated sera from patients with neurocysticercosis with or without complement (guinea pig serum). Controls included oncospheres plus complement alone, normal human serum alone, normal serum with complement, or buffer alone. Serum from infected patients, especially with complement, markedly reduced oncosphere mobility and led to disappearance of secretory vesicles and loss of membrane integrity. Viability as assessed by staining with dimethyl-thiazolyl-diphenyl-tetrazolium was reduced from 92.5% in controls to 61.5% with immune serum and 38.8% with immune serum and complement (P < 0.01). Preliminary western blot analysis showed antigens at 22, 64, and 70 kDa recognized by all 3 sera, but not by control sera. These data suggest that sera from patients with cysticercosis can kill oncospheres in vitro and may be used to identify protective antigens.

Original languageEnglish (US)
Pages (from-to)124-127
Number of pages4
JournalJournal of Parasitology
Volume79
Issue number1
StatePublished - 1993
Externally publishedYes

Fingerprint

neurocysticercosis
oncospheres
Taenia solium
Neurocysticercosis
blood serum
serum
viability
complement
Serum
antiserum
Immune Sera
Immunity
immunity
antigen
antigens
Antigens
Cysticercosis
cysticercosis
Parasitic Diseases
effect

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology
  • Parasitology

Cite this

Effects of serum from Neurocysticercosis patients on the structure and viability of Taenia solium oncospheres. / Molinari, J. L.; Tato, P.; Lara-Aguilera, R.; White, A. Clinton.

In: Journal of Parasitology, Vol. 79, No. 1, 1993, p. 124-127.

Research output: Contribution to journalArticle

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