TY - JOUR
T1 - Effects of Topical Antimicrobial Agents on the Human Neutrophil Respiratory Burst
AU - Hansbrough, John F.
AU - Zapata Sirvent, Ramon L.
AU - Cooper, Matthew L.
PY - 1991/5
Y1 - 1991/5
N2 - The neutrophil oxidative burst plays an important role in killing intracellular microorganisms. We studied the effects of topical antimicrobial agents on the N-formyl-l-methionyl-l-leucyl-l-phenylalanine–stimulated oxidative burst of human peripheral blood neutrophils, using a flow cytometric assay. Mafenide acetate, sulfadiazine silver, gentamicin sulfate, neomycin sulfate–polymyxin B sulfate (Neosporin GU Irrigant), acetic acid, amphotericin B, and povidone-iodine inhibited the neutrophil oxidative burst at or below clinical concentrations, while 0.25% modified diluted sodium hypochlorite (Dakin's) solution caused cell death. Bacitracin–polymyxin B sulfate (Polysporin) greatly augmented the respiratory burst; this effect was due to the bacitracin component. Diluted gentamicin and acetic acid also augmented intracellular hydrogen peroxide production, but to a lesser extent than Polysporin. Inhibition of the respiratory burst of neutrophils might be considered when these agents are used for topical wound care, although clinical correlates of these effects have not been determined.
AB - The neutrophil oxidative burst plays an important role in killing intracellular microorganisms. We studied the effects of topical antimicrobial agents on the N-formyl-l-methionyl-l-leucyl-l-phenylalanine–stimulated oxidative burst of human peripheral blood neutrophils, using a flow cytometric assay. Mafenide acetate, sulfadiazine silver, gentamicin sulfate, neomycin sulfate–polymyxin B sulfate (Neosporin GU Irrigant), acetic acid, amphotericin B, and povidone-iodine inhibited the neutrophil oxidative burst at or below clinical concentrations, while 0.25% modified diluted sodium hypochlorite (Dakin's) solution caused cell death. Bacitracin–polymyxin B sulfate (Polysporin) greatly augmented the respiratory burst; this effect was due to the bacitracin component. Diluted gentamicin and acetic acid also augmented intracellular hydrogen peroxide production, but to a lesser extent than Polysporin. Inhibition of the respiratory burst of neutrophils might be considered when these agents are used for topical wound care, although clinical correlates of these effects have not been determined.
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U2 - 10.1001/archsurg.1991.01410290079016
DO - 10.1001/archsurg.1991.01410290079016
M3 - Article
C2 - 1850590
AN - SCOPUS:0025891235
SN - 0004-0010
VL - 126
SP - 603
EP - 608
JO - Archives of Surgery
JF - Archives of Surgery
IS - 5
ER -