Effects of Topical Antimicrobial Agents on the Human Neutrophil Respiratory Burst

John F. Hansbrough, Ramon Zapata Sirvent, Matthew L. Cooper

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The neutrophil oxidative burst plays an important role in killing intracellular microorganisms. We studied the effects of topical antimicrobial agents on the N-formyl-l-methionyl-l-leucyl-l-phenylalanine–stimulated oxidative burst of human peripheral blood neutrophils, using a flow cytometric assay. Mafenide acetate, sulfadiazine silver, gentamicin sulfate, neomycin sulfate–polymyxin B sulfate (Neosporin GU Irrigant), acetic acid, amphotericin B, and povidone-iodine inhibited the neutrophil oxidative burst at or below clinical concentrations, while 0.25% modified diluted sodium hypochlorite (Dakin's) solution caused cell death. Bacitracin–polymyxin B sulfate (Polysporin) greatly augmented the respiratory burst; this effect was due to the bacitracin component. Diluted gentamicin and acetic acid also augmented intracellular hydrogen peroxide production, but to a lesser extent than Polysporin. Inhibition of the respiratory burst of neutrophils might be considered when these agents are used for topical wound care, although clinical correlates of these effects have not been determined.

Original languageEnglish (US)
Pages (from-to)603-608
Number of pages6
JournalArchives of Surgery
Volume126
Issue number5
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Respiratory Burst
Anti-Infective Agents
Neutrophils
Gentamicins
Acetic Acid
Mafenide
Framycetin
Silver Sulfadiazine
Bacitracin
Povidone-Iodine
Sodium Hypochlorite
Amphotericin B
Hydrogen Peroxide
Sulfates
Cell Death
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Effects of Topical Antimicrobial Agents on the Human Neutrophil Respiratory Burst. / Hansbrough, John F.; Zapata Sirvent, Ramon; Cooper, Matthew L.

In: Archives of Surgery, Vol. 126, No. 5, 01.01.1991, p. 603-608.

Research output: Contribution to journalArticle

Hansbrough, John F. ; Zapata Sirvent, Ramon ; Cooper, Matthew L. / Effects of Topical Antimicrobial Agents on the Human Neutrophil Respiratory Burst. In: Archives of Surgery. 1991 ; Vol. 126, No. 5. pp. 603-608.
@article{166419a8afa94b039fb76cac3998c36a,
title = "Effects of Topical Antimicrobial Agents on the Human Neutrophil Respiratory Burst",
abstract = "The neutrophil oxidative burst plays an important role in killing intracellular microorganisms. We studied the effects of topical antimicrobial agents on the N-formyl-l-methionyl-l-leucyl-l-phenylalanine–stimulated oxidative burst of human peripheral blood neutrophils, using a flow cytometric assay. Mafenide acetate, sulfadiazine silver, gentamicin sulfate, neomycin sulfate–polymyxin B sulfate (Neosporin GU Irrigant), acetic acid, amphotericin B, and povidone-iodine inhibited the neutrophil oxidative burst at or below clinical concentrations, while 0.25{\%} modified diluted sodium hypochlorite (Dakin's) solution caused cell death. Bacitracin–polymyxin B sulfate (Polysporin) greatly augmented the respiratory burst; this effect was due to the bacitracin component. Diluted gentamicin and acetic acid also augmented intracellular hydrogen peroxide production, but to a lesser extent than Polysporin. Inhibition of the respiratory burst of neutrophils might be considered when these agents are used for topical wound care, although clinical correlates of these effects have not been determined.",
author = "Hansbrough, {John F.} and {Zapata Sirvent}, Ramon and Cooper, {Matthew L.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1001/archsurg.1991.01410290079016",
language = "English (US)",
volume = "126",
pages = "603--608",
journal = "JAMA Surgery",
issn = "2168-6254",
publisher = "American Medical Association",
number = "5",

}

TY - JOUR

T1 - Effects of Topical Antimicrobial Agents on the Human Neutrophil Respiratory Burst

AU - Hansbrough, John F.

AU - Zapata Sirvent, Ramon

AU - Cooper, Matthew L.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The neutrophil oxidative burst plays an important role in killing intracellular microorganisms. We studied the effects of topical antimicrobial agents on the N-formyl-l-methionyl-l-leucyl-l-phenylalanine–stimulated oxidative burst of human peripheral blood neutrophils, using a flow cytometric assay. Mafenide acetate, sulfadiazine silver, gentamicin sulfate, neomycin sulfate–polymyxin B sulfate (Neosporin GU Irrigant), acetic acid, amphotericin B, and povidone-iodine inhibited the neutrophil oxidative burst at or below clinical concentrations, while 0.25% modified diluted sodium hypochlorite (Dakin's) solution caused cell death. Bacitracin–polymyxin B sulfate (Polysporin) greatly augmented the respiratory burst; this effect was due to the bacitracin component. Diluted gentamicin and acetic acid also augmented intracellular hydrogen peroxide production, but to a lesser extent than Polysporin. Inhibition of the respiratory burst of neutrophils might be considered when these agents are used for topical wound care, although clinical correlates of these effects have not been determined.

AB - The neutrophil oxidative burst plays an important role in killing intracellular microorganisms. We studied the effects of topical antimicrobial agents on the N-formyl-l-methionyl-l-leucyl-l-phenylalanine–stimulated oxidative burst of human peripheral blood neutrophils, using a flow cytometric assay. Mafenide acetate, sulfadiazine silver, gentamicin sulfate, neomycin sulfate–polymyxin B sulfate (Neosporin GU Irrigant), acetic acid, amphotericin B, and povidone-iodine inhibited the neutrophil oxidative burst at or below clinical concentrations, while 0.25% modified diluted sodium hypochlorite (Dakin's) solution caused cell death. Bacitracin–polymyxin B sulfate (Polysporin) greatly augmented the respiratory burst; this effect was due to the bacitracin component. Diluted gentamicin and acetic acid also augmented intracellular hydrogen peroxide production, but to a lesser extent than Polysporin. Inhibition of the respiratory burst of neutrophils might be considered when these agents are used for topical wound care, although clinical correlates of these effects have not been determined.

UR - http://www.scopus.com/inward/record.url?scp=0025891235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025891235&partnerID=8YFLogxK

U2 - 10.1001/archsurg.1991.01410290079016

DO - 10.1001/archsurg.1991.01410290079016

M3 - Article

VL - 126

SP - 603

EP - 608

JO - JAMA Surgery

JF - JAMA Surgery

SN - 2168-6254

IS - 5

ER -