Effects of tributyltin on placental cytokine production

Yuko Arita, Michael Kirk, Neha Gupta, Ramkumar Menon, Darios Getahun, Morgan R. Peltier

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tributyltin (TBT) is a persistent pollutant but its effects on placental function are poorly understood as are its possible interactions with infection. We hypothesized that TBT alters the production of sex hormones and biomarkers for inflammation and neurodevelopment in an infection-dependent manner. Placental explant cultures were treated with 0-5000 nM TBT in the presence and absence of Escherichia coli. A conditioned medium was harvested and concentrations of steroids (progesterone, P4; testosterone, T and estradiol, E2) as well as biomarkers of inflammation [interleukin (IL)-1β (IL-1β), tumor necrosis factor (TNF-α), IL-10, IL-6, soluble glycoprotein 130 (sgp-130) and heme oxygenase-1 (HO-1)], oxidative stress [8-iso-prostaglandin (8-IsoP)] and neurodevelopment [brain-derived neurotrophic factor (BDNF)] were quantified. TBT increased P4 slightly but had little or no effect on T or E2 production. IL-1β, IL-6, sgp-130, IL-10 and 8-IsoP production was enhanced by TBT. P4 and IL-6 production was also enhanced by TBT for bacteria-stimulated cultures but TBT significantly inhibited bacteria-induced IL-1β and sgp-130 production. High doses of TBT also inhibited BDNF production. TBT increases P4 but has minimal effect on downstream steroids. It enhances the production of inflammatory biomarkers such as IL-1β, TNF-α, IL-10 and IL-6. Inhibition of sgp-130 by TBT suggests that TBT may increase bioactive IL-6 production which has been associated with adverse neurodevelopmental outcomes. Reduced expression of BDNF also supports this possibility.

Original languageEnglish (US)
JournalUnknown Journal
DOIs
StateAccepted/In press - Mar 15 2018

Fingerprint

Cytokines
Cytokine Receptor gp130
Interleukin-6
Interleukin-1
Brain-Derived Neurotrophic Factor
Interleukin-10
Biomarkers
Prostaglandins
tributyltin
Steroids
Inflammation
Bacteria
Heme Oxygenase-1
Gonadal Steroid Hormones
Conditioned Culture Medium
Infection
Interleukin-8
Progesterone
Testosterone
Estradiol

Keywords

  • Autism
  • cytokine
  • environmental pollutants
  • infection
  • placenta
  • preterm birth
  • tributyltin

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Arita, Y., Kirk, M., Gupta, N., Menon, R., Getahun, D., & Peltier, M. R. (Accepted/In press). Effects of tributyltin on placental cytokine production. Unknown Journal. https://doi.org/10.1515/jpm-2017-0336

Effects of tributyltin on placental cytokine production. / Arita, Yuko; Kirk, Michael; Gupta, Neha; Menon, Ramkumar; Getahun, Darios; Peltier, Morgan R.

In: Unknown Journal, 15.03.2018.

Research output: Contribution to journalArticle

Arita, Yuko ; Kirk, Michael ; Gupta, Neha ; Menon, Ramkumar ; Getahun, Darios ; Peltier, Morgan R. / Effects of tributyltin on placental cytokine production. In: Unknown Journal. 2018.
@article{4014dfec2b1b4e2dae22e5d8abd8258f,
title = "Effects of tributyltin on placental cytokine production",
abstract = "Tributyltin (TBT) is a persistent pollutant but its effects on placental function are poorly understood as are its possible interactions with infection. We hypothesized that TBT alters the production of sex hormones and biomarkers for inflammation and neurodevelopment in an infection-dependent manner. Placental explant cultures were treated with 0-5000 nM TBT in the presence and absence of Escherichia coli. A conditioned medium was harvested and concentrations of steroids (progesterone, P4; testosterone, T and estradiol, E2) as well as biomarkers of inflammation [interleukin (IL)-1β (IL-1β), tumor necrosis factor (TNF-α), IL-10, IL-6, soluble glycoprotein 130 (sgp-130) and heme oxygenase-1 (HO-1)], oxidative stress [8-iso-prostaglandin (8-IsoP)] and neurodevelopment [brain-derived neurotrophic factor (BDNF)] were quantified. TBT increased P4 slightly but had little or no effect on T or E2 production. IL-1β, IL-6, sgp-130, IL-10 and 8-IsoP production was enhanced by TBT. P4 and IL-6 production was also enhanced by TBT for bacteria-stimulated cultures but TBT significantly inhibited bacteria-induced IL-1β and sgp-130 production. High doses of TBT also inhibited BDNF production. TBT increases P4 but has minimal effect on downstream steroids. It enhances the production of inflammatory biomarkers such as IL-1β, TNF-α, IL-10 and IL-6. Inhibition of sgp-130 by TBT suggests that TBT may increase bioactive IL-6 production which has been associated with adverse neurodevelopmental outcomes. Reduced expression of BDNF also supports this possibility.",
keywords = "Autism, cytokine, environmental pollutants, infection, placenta, preterm birth, tributyltin",
author = "Yuko Arita and Michael Kirk and Neha Gupta and Ramkumar Menon and Darios Getahun and Peltier, {Morgan R.}",
year = "2018",
month = "3",
day = "15",
doi = "10.1515/jpm-2017-0336",
language = "English (US)",
journal = "Molecular Oncology",
issn = "1574-7891",
publisher = "Elsevier",

}

TY - JOUR

T1 - Effects of tributyltin on placental cytokine production

AU - Arita, Yuko

AU - Kirk, Michael

AU - Gupta, Neha

AU - Menon, Ramkumar

AU - Getahun, Darios

AU - Peltier, Morgan R.

PY - 2018/3/15

Y1 - 2018/3/15

N2 - Tributyltin (TBT) is a persistent pollutant but its effects on placental function are poorly understood as are its possible interactions with infection. We hypothesized that TBT alters the production of sex hormones and biomarkers for inflammation and neurodevelopment in an infection-dependent manner. Placental explant cultures were treated with 0-5000 nM TBT in the presence and absence of Escherichia coli. A conditioned medium was harvested and concentrations of steroids (progesterone, P4; testosterone, T and estradiol, E2) as well as biomarkers of inflammation [interleukin (IL)-1β (IL-1β), tumor necrosis factor (TNF-α), IL-10, IL-6, soluble glycoprotein 130 (sgp-130) and heme oxygenase-1 (HO-1)], oxidative stress [8-iso-prostaglandin (8-IsoP)] and neurodevelopment [brain-derived neurotrophic factor (BDNF)] were quantified. TBT increased P4 slightly but had little or no effect on T or E2 production. IL-1β, IL-6, sgp-130, IL-10 and 8-IsoP production was enhanced by TBT. P4 and IL-6 production was also enhanced by TBT for bacteria-stimulated cultures but TBT significantly inhibited bacteria-induced IL-1β and sgp-130 production. High doses of TBT also inhibited BDNF production. TBT increases P4 but has minimal effect on downstream steroids. It enhances the production of inflammatory biomarkers such as IL-1β, TNF-α, IL-10 and IL-6. Inhibition of sgp-130 by TBT suggests that TBT may increase bioactive IL-6 production which has been associated with adverse neurodevelopmental outcomes. Reduced expression of BDNF also supports this possibility.

AB - Tributyltin (TBT) is a persistent pollutant but its effects on placental function are poorly understood as are its possible interactions with infection. We hypothesized that TBT alters the production of sex hormones and biomarkers for inflammation and neurodevelopment in an infection-dependent manner. Placental explant cultures were treated with 0-5000 nM TBT in the presence and absence of Escherichia coli. A conditioned medium was harvested and concentrations of steroids (progesterone, P4; testosterone, T and estradiol, E2) as well as biomarkers of inflammation [interleukin (IL)-1β (IL-1β), tumor necrosis factor (TNF-α), IL-10, IL-6, soluble glycoprotein 130 (sgp-130) and heme oxygenase-1 (HO-1)], oxidative stress [8-iso-prostaglandin (8-IsoP)] and neurodevelopment [brain-derived neurotrophic factor (BDNF)] were quantified. TBT increased P4 slightly but had little or no effect on T or E2 production. IL-1β, IL-6, sgp-130, IL-10 and 8-IsoP production was enhanced by TBT. P4 and IL-6 production was also enhanced by TBT for bacteria-stimulated cultures but TBT significantly inhibited bacteria-induced IL-1β and sgp-130 production. High doses of TBT also inhibited BDNF production. TBT increases P4 but has minimal effect on downstream steroids. It enhances the production of inflammatory biomarkers such as IL-1β, TNF-α, IL-10 and IL-6. Inhibition of sgp-130 by TBT suggests that TBT may increase bioactive IL-6 production which has been associated with adverse neurodevelopmental outcomes. Reduced expression of BDNF also supports this possibility.

KW - Autism

KW - cytokine

KW - environmental pollutants

KW - infection

KW - placenta

KW - preterm birth

KW - tributyltin

UR - http://www.scopus.com/inward/record.url?scp=85045508342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045508342&partnerID=8YFLogxK

U2 - 10.1515/jpm-2017-0336

DO - 10.1515/jpm-2017-0336

M3 - Article

JO - Molecular Oncology

JF - Molecular Oncology

SN - 1574-7891

ER -