Effects of ultrasound-targeted microbubble destruction on cardiac gene expression

Raffi Bekeredjian, Shuyuan Chen, Wentong Pan, Paul A. Grayburn, Ralph V. Shohet

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Ultrasound (US) contrast agents are increasingly used in diagnostic echocardiography. Recent studies have suggested unanticipated effects of microbubble destruction. This study was designed to evaluate gene regulation caused by US-mediated destruction of microbubbles in the heart. During IV infusion of Optison™, triggered US was applied to rat hearts to destroy microbubbles. A control group received only saline and US. RNA was isolated from hearts 24 and 72 h after treatment. Analysis with a deeply representative murine cardiac-specific microarray was used to identify regulated genes. Real-time polymerase chain reaction (PCR) was then applied to verify regulated genes. Microarray analysis revealed only 5 regulated genes in the 24-h group and 4 in the 72-h group. Of these genes, only carbonic anhydrase was significantly upregulated in the 24-h Optison™ group (4.3 fold; p = 0.0005) when examined in individual animals by real-time PCR. By this very sensitive technique, the bioeffects of microbubble destruction are negligible.

Original languageEnglish (US)
Pages (from-to)539-543
Number of pages5
JournalUltrasound in Medicine and Biology
Volume30
Issue number4
DOIs
StatePublished - Apr 1 2004
Externally publishedYes

Keywords

  • Echocardiography
  • Gene regulation
  • Microarray
  • Microbubbles

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Biophysics
  • Acoustics and Ultrasonics

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