Efficacy of genital T cell responses to herpes simplex virus type 2 resulting from immunization of the nasal mucosa

Gregg N. Milligan, Kristen L. Dudley-McClain, Chin Fun Chu, Christal G. Young

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Intravaginal (ivag) or intranasal (i.n.) immunization of C57BL/6J (B6) mice with a thymidine kinase-deficient strain (tk-) of herpes simplex virus type 2 (HSV-2) resulted in comparable protection of the genital epithelium and sensory ganglia against HSV-2 challenge. In contrast, protection of these sites was much reduced in i.n.-immunized compared to ivag-immunized B cell-deficient μMT mice. Fewer HSV-specific T cells were detected in the genital epithelium of i.n.-immunized compared to ivag-immunized μMT mice after HSV-2 challenge. Passive transfer of HSV-specific serum to immune μMT mice restored protection of these sites against HSV-2 challenge. These results suggest that protection of genital and neuronal sites may be conferred by i.n. immunization but may be more dependent on antibody-dependent mechanisms than the protection resulting from genital immunization. These results have implications for immunization strategies to elicit high levels of cell-mediated protection of the genital tract and sensory ganglia.

Original languageEnglish (US)
Pages (from-to)507-515
Number of pages9
JournalVirology
Volume318
Issue number2
DOIs
StatePublished - Jan 20 2004

Keywords

  • Genital tract
  • Herpes simplex virus
  • Sensory ganglia
  • T lymphocyte

ASJC Scopus subject areas

  • Virology

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