Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Polη

Robert E. Johnson, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

643 Scopus citations

Abstract

The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine- thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase η.

Original languageEnglish (US)
Pages (from-to)1001-1004
Number of pages4
JournalScience
Volume283
Issue number5404
DOIs
StatePublished - Feb 12 1999

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