Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Polη

Robert E. Johnson, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

636 Citations (Scopus)

Abstract

The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine- thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase η.

Original languageEnglish (US)
Pages (from-to)1001-1004
Number of pages4
JournalScience
Volume283
Issue number5404
DOIs
StatePublished - Feb 12 1999

Fingerprint

Pyrimidine Dimers
Thymine
DNA-Directed DNA Polymerase
Yeasts
Cyclobutanes
Adenine
DNA Repair
Saccharomyces cerevisiae
Nucleotides
Genes

ASJC Scopus subject areas

  • General

Cite this

Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Polη. / Johnson, Robert E.; Prakash, Satya; Prakash, Louise.

In: Science, Vol. 283, No. 5404, 12.02.1999, p. 1001-1004.

Research output: Contribution to journalArticle

@article{1d32f6261b3c47e493ac213054acf5a0,
title = "Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Polη",
abstract = "The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine- thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase η.",
author = "Johnson, {Robert E.} and Satya Prakash and Louise Prakash",
year = "1999",
month = "2",
day = "12",
doi = "10.1126/science.283.5404.1001",
language = "English (US)",
volume = "283",
pages = "1001--1004",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5404",

}

TY - JOUR

T1 - Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Polη

AU - Johnson, Robert E.

AU - Prakash, Satya

AU - Prakash, Louise

PY - 1999/2/12

Y1 - 1999/2/12

N2 - The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine- thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase η.

AB - The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine- thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase η.

UR - http://www.scopus.com/inward/record.url?scp=0033548231&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033548231&partnerID=8YFLogxK

U2 - 10.1126/science.283.5404.1001

DO - 10.1126/science.283.5404.1001

M3 - Article

C2 - 9974380

AN - SCOPUS:0033548231

VL - 283

SP - 1001

EP - 1004

JO - Science

JF - Science

SN - 0036-8075

IS - 5404

ER -