EIA with species-specific monoclonal antibodies: A novel seroepidemiologic tool for determination of the etiologic agent of spotted fever rickettsiosis

David Walker, Robert Speed, Hui Min Feng, Catherine Taylor, David H. Walker

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

All currently available assays for antibodies to the etiologic agents of spotted fever group rickettsioses detect reactivity with antigens of lipopolysaccharides and the major cell wall proteins which contain epitopes that are shared among many spotted fever group rickettsiae. The hypothesis of this study was that a monoclonal antibody to a species-specific epitope of Rickettsia rickettsii would be blocked from binding to the rickettsial surface if the rickettsiae were incubated previously with serum containing species-specific antibodies to the epitope. In an EIA, binding of monoclonal antibody 5C10-F3 to R. rickettsii was blocked by convalescent sera from patients with Rocky Mountain spotted fever but not from those with Mediterranean spotted fever, endemic typhus, or Q fever or normal subjects. This assay should be useful in determining the origin of the high seroprevalence of spotted fever rickettsial antibodies in certain geographic regions and establishing a species-specific diagnosis in patients with undetermined rickettsial exposure.

Original languageEnglish (US)
Pages (from-to)1292-1295
Number of pages4
JournalJournal of Infectious Diseases
Volume168
Issue number5
StatePublished - Nov 1993

Fingerprint

Rickettsia rickettsii
Epitopes
Rickettsia
Fever
Monoclonal Antibodies
Antibodies
Endemic Flea-Borne Typhus
Boutonneuse Fever
Rocky Mountain Spotted Fever
Q Fever
Seroepidemiologic Studies
Serum
Cell Wall
Lipopolysaccharides
Antigens
Proteins

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

EIA with species-specific monoclonal antibodies : A novel seroepidemiologic tool for determination of the etiologic agent of spotted fever rickettsiosis. / Walker, David; Speed, Robert; Feng, Hui Min; Taylor, Catherine; Walker, David H.

In: Journal of Infectious Diseases, Vol. 168, No. 5, 11.1993, p. 1292-1295.

Research output: Contribution to journalArticle

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