Eilat virus induces both homologous and heterologous interference

Farooq Nasar, Jesse H. Erasmus, Andrew D. Haddow, Robert B. Tesh, Scott Weaver

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Most alphaviruses are mosquito-borne and exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, and humans. Occasionally, alphaviruses can spill over into the human population and cause disease characterized by debilitating arthralgia or fatal encephalitis. Recently, a unique alphavirus, Eilat virus (EILV), was described that readily infects mosquito but not vertebrate cell lines. Here, we investigated the ability of EILV to induce superinfection exclusion. Prior infection of C7/10 (. Aedes albopictus) cells with EILV induced homologous and heterologous interference, reducing the virus titers of heterologous superinfecting viruses (SINV, VEEV, EEEV, WEEV, and CHIKV) by ~10-10,000 fold and delaying replication kinetics by 12-48. h. Similar to in vitro infection, prior in vivo EILV infection of Aedes aegypti mosquitoes delayed dissemination of chikungunya virus for 3 days. This is the first evidence of heterologous interference induced by a mosquito-specific alphavirus in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)51-58
Number of pages8
JournalVirology
Volume484
DOIs
StatePublished - Oct 1 2015

Fingerprint

Alphavirus
Culicidae
Viruses
Aedes
Vertebrates
Chikungunya virus
Superinfection
Aptitude
Host Specificity
Arthralgia
Virus Diseases
Encephalitis
Infection
Viral Load
Birds
Rodentia
Cell Line
Population
In Vitro Techniques

Keywords

  • Alphavirus
  • Eilat virus
  • Superinfection

ASJC Scopus subject areas

  • Virology

Cite this

Eilat virus induces both homologous and heterologous interference. / Nasar, Farooq; Erasmus, Jesse H.; Haddow, Andrew D.; Tesh, Robert B.; Weaver, Scott.

In: Virology, Vol. 484, 01.10.2015, p. 51-58.

Research output: Contribution to journalArticle

Nasar, Farooq ; Erasmus, Jesse H. ; Haddow, Andrew D. ; Tesh, Robert B. ; Weaver, Scott. / Eilat virus induces both homologous and heterologous interference. In: Virology. 2015 ; Vol. 484. pp. 51-58.
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