Electrical properties of acutely isolated, identified rat spinal dorsal horn projection neurons

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    20 Scopus citations

    Abstract

    We used a retrograde marker, rhodamine-labeled fluorescent latex microspheres and the enzyme papain to isolate a group of identified, dorsal horn projection neurons from rat spinal cord. This method has allowed us to study the electrical properties of these acutely dissociated cells with patch-clamp techniques. It will also provide us the opportunity to understand the transmitter responses of these neurons that had not undergone culturing manipulation. The isolated projection cells have a short-lasting action potential and fire repetitively under a sustained depolarization. The ionic conductance mechanisms underlying the action potentials were studied under voltage-clamp conditions. Voltage-dependent inward sodium, inward calcium, outward potassium and calcium-activated potassium currents were observed in these cells.

    Original languageEnglish (US)
    Pages (from-to)267-272
    Number of pages6
    JournalNeuroscience Letters
    Volume82
    Issue number3
    DOIs
    StatePublished - Dec 4 1987

    Keywords

    • Acutely isolated spinal cord cell
    • Ca channel
    • K channel
    • Na channel
    • Patch-clamp
    • Spinothalamic neuron
    • Trigeminothalamic neuron

    ASJC Scopus subject areas

    • General Neuroscience

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