Electrical properties of acutely isolated, identified rat spinal dorsal horn projection neurons

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Abstract

We used a retrograde marker, rhodamine-labeled fluorescent latex microspheres and the enzyme papain to isolate a group of identified, dorsal horn projection neurons from rat spinal cord. This method has allowed us to study the electrical properties of these acutely dissociated cells with patch-clamp techniques. It will also provide us the opportunity to understand the transmitter responses of these neurons that had not undergone culturing manipulation. The isolated projection cells have a short-lasting action potential and fire repetitively under a sustained depolarization. The ionic conductance mechanisms underlying the action potentials were studied under voltage-clamp conditions. Voltage-dependent inward sodium, inward calcium, outward potassium and calcium-activated potassium currents were observed in these cells.

Original languageEnglish (US)
Pages (from-to)267-272
Number of pages6
JournalNeuroscience Letters
Volume82
Issue number3
DOIs
StatePublished - Dec 4 1987

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Keywords

  • Acutely isolated spinal cord cell
  • Ca channel
  • K channel
  • Na channel
  • Patch-clamp
  • Spinothalamic neuron
  • Trigeminothalamic neuron

ASJC Scopus subject areas

  • Neuroscience(all)

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