Electrochemical monitoring of superoxide anion production and cerebral blood flow: Effect of interleukin-1β pretreatment in a model of focal ischemia and reperfusion

Roderic H. Fabian, J. Regino Perez-Polo, Thomas A. Kent

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Conditions associated with systemic infection, such as endotoxinemia, are known to increase the levels of proinflammatory cytokines such as interleukin (IL)-1 in the central nervous system. Systemic infection has been shown to be a common preexisting condition in patients with stroke. To examine a possible consequence of systemic infection, we used a novel electrochemical technique, which combines measurement of cerebral blood flow with measurement of superoxide anion concentrations, to examine the effect of pretreatment of pial vasculature with a proinflammatory cytokine, IL-1β, on cerebral blood flow and superoxide anion concentration in a rat model of middle cerebral artery occlusion and reperfusion. In addition, neutrophil recruitment was measured using an immunohistochemical technique. Our results indicate that exposure of pial and cerebral vasculature to IL-1β significantly accelerates recruitment of neutrophils, reduces cerebral blood flow, and increases superoxide anion concentration at the pial surface during reperfusion. These results support the idea that prior exposure of brain vasculature to IL-1β results in acceleration of cerebrovascular injury by accelerating recruitment of neutrophils, which secrete superoxide anion, during reperfusion. This finding has possible implications for the treatment of stroke with reperfusion agents in patients with preexisting infections. (C) 2000 Wiley-Liss, Inc.

    Original languageEnglish (US)
    Pages (from-to)795-803
    Number of pages9
    JournalJournal of Neuroscience Research
    Volume60
    Issue number6
    DOIs
    StatePublished - Jun 15 2000

    Fingerprint

    Cerebrovascular Circulation
    Interleukin-1
    Superoxides
    Reperfusion
    Neutrophil Infiltration
    Ischemia
    Infection
    Stroke
    Electrochemical Techniques
    Cytokines
    Preexisting Condition Coverage
    Middle Cerebral Artery Infarction
    Proxy
    Central Nervous System
    Wounds and Injuries
    Brain

    Keywords

    • Cerebral blood flow
    • Cerebral ischemia
    • Cytokines
    • Neutrophil
    • Superoxide

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Electrochemical monitoring of superoxide anion production and cerebral blood flow : Effect of interleukin-1β pretreatment in a model of focal ischemia and reperfusion. / Fabian, Roderic H.; Perez-Polo, J. Regino; Kent, Thomas A.

    In: Journal of Neuroscience Research, Vol. 60, No. 6, 15.06.2000, p. 795-803.

    Research output: Contribution to journalArticle

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