Electron Beam Coater for Reduction of Charging in Ice-Embedded Biological Specimens using Ti88Si12 Alloy

Michael Sherman, Wah Chiu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biological macromolecules embedded in vitreous ice are known to suffer from charging while being imaged in an electron transmission cryomicroscope. We developed an electron beam coater that deposits conductive films onto the surface of frozen-hydrated specimens. The conductive films help to dissipate charge during electron irradiation of poorly conductive ice-embedded biological samples. We observed significant reduction in charging of ice-embedded catalase crystals suspended over holes in a holey carbon film after coating them with a 30-Å-thick layer of an amorphous alloy, Ti88Si12. Images of the crystals after coating showed diffraction spots of up to 3 Å resolution.

Original languageEnglish (US)
Pages (from-to)566-573
Number of pages8
JournalMicroscopy and Microanalysis
Volume9
Issue number6
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Ice
charging
Electron beams
ice
Conductive films
electron beams
coating
catalase
Coatings
Crystals
Electron irradiation
Carbon films
Amorphous alloys
electron irradiation
Macromolecules
macromolecules
crystals
Deposits
Diffraction
deposits

Keywords

  • Charging
  • Conductive coating
  • Electron cryomicroscopy

ASJC Scopus subject areas

  • Instrumentation

Cite this

Electron Beam Coater for Reduction of Charging in Ice-Embedded Biological Specimens using Ti88Si12 Alloy. / Sherman, Michael; Chiu, Wah.

In: Microscopy and Microanalysis, Vol. 9, No. 6, 12.2003, p. 566-573.

Research output: Contribution to journalArticle

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