Electron capture dissociation and infrared multiphoton dissociation MS/MS of an N-glycosylated tryptic peptide to yield complementary sequence information

K. Håkansson, H. J. Cooper, Mark Emmett, C. E. Costello, A. G. Marshall, C. L. Nilsson

Research output: Contribution to journalArticle

327 Citations (Scopus)

Abstract

Glycoproteins are a functionally important class of biomolecules for which structural elucidation presents a challenge. Fragmentation of N-glycosylated peptides, employing collisionally activated dissociation, typically yields product ions that result from dissociation at glycosidic bonds, with little occurrence of dissociation at peptide backbone sites. We have applied two dissociation techniques, electron capture dissociation (ECD) and infrared multiphoton dissociation (IRMPD), in a 7-T Fourier transform ion cyclotron resonance mass spectrometer, in the investigation of an N-glycosylated peptide from an unfractionated tryptic digest of the lectin of the coral tree, Erythrina corallodendron. ECD provided c and z ions derived from the peptide backbone, with no observed loss of sugars. Cleavage at 11 of 15 backbone amine bonds was observed. The lack of cleavage at sites located close to the glycosylated asparagine residue may result from steric blocking by the glycan. IRMPD provided abundant fragment ions, primarily through dissociation at glycosidic linkages. The monosaccharide composition and the presence of three glycan branch sites could be determined from the IRMPD fragments. The two types of spectra, obtained with the same instrument, thus provide complementary structural information about the glycopeptide. The current result extends the applicability of ECD for glycopeptide analysis to N-glycosylated peptides and to peptides containing branched, highly substituted glycans.

Original languageEnglish (US)
Pages (from-to)4530-4536
Number of pages7
JournalAnalytical Chemistry
Volume73
Issue number18
DOIs
StatePublished - Sep 15 2001
Externally publishedYes

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Infrared radiation
Peptides
Electrons
Ions
Polysaccharides
Glycopeptides
Cyclotron resonance
Monosaccharides
Asparagine
Biomolecules
Mass spectrometers
Lectins
Sugars
Amines
Glycoproteins
Fourier transforms
Chemical analysis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Electron capture dissociation and infrared multiphoton dissociation MS/MS of an N-glycosylated tryptic peptide to yield complementary sequence information. / Håkansson, K.; Cooper, H. J.; Emmett, Mark; Costello, C. E.; Marshall, A. G.; Nilsson, C. L.

In: Analytical Chemistry, Vol. 73, No. 18, 15.09.2001, p. 4530-4536.

Research output: Contribution to journalArticle

Håkansson, K. ; Cooper, H. J. ; Emmett, Mark ; Costello, C. E. ; Marshall, A. G. ; Nilsson, C. L. / Electron capture dissociation and infrared multiphoton dissociation MS/MS of an N-glycosylated tryptic peptide to yield complementary sequence information. In: Analytical Chemistry. 2001 ; Vol. 73, No. 18. pp. 4530-4536.
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