Electron capture dissociation: Optimization, mechanism, and applications

A. G. Marshall, M. J. Chalmers, H. J. Cooper, Mark Emmett, K. Håkansson, C. L. Hendrickson, R. R. Hudgins, C. L. Nilsson, J. P. Quinn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The application of electron capture dissociation (ECD) as an analytical method for determining peptide and protein primary amino acid sequences as well as post-translational modification sites is discussed. A 7.0 and 9.4 T homebuilt Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers were used to conduct ECD. Multiply-charged cations were generated by microelectrospray ionization and to permit infrared multiphoton dissociation (IRMPD) an ECD dispenser electrode was positioned off-axis. The results show amino acid side-chain cleavages for arginine, histidine, asparagine/glutamine, methionine and lysine residues.

Original languageEnglish
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages107-108
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Electrons
Dispensers
Amino Acids
Cyclotron resonance
Asparagine
Mass spectrometers
Glutamine
Histidine
Methionine
Lysine
Ionization
Arginine
Cations
Fourier transforms
Ions
Infrared radiation
Peptides
Electrodes
Proteins

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Marshall, A. G., Chalmers, M. J., Cooper, H. J., Emmett, M., Håkansson, K., Hendrickson, C. L., ... Quinn, J. P. (2002). Electron capture dissociation: Optimization, mechanism, and applications. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 107-108)

Electron capture dissociation : Optimization, mechanism, and applications. / Marshall, A. G.; Chalmers, M. J.; Cooper, H. J.; Emmett, Mark; Håkansson, K.; Hendrickson, C. L.; Hudgins, R. R.; Nilsson, C. L.; Quinn, J. P.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 107-108.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Marshall, AG, Chalmers, MJ, Cooper, HJ, Emmett, M, Håkansson, K, Hendrickson, CL, Hudgins, RR, Nilsson, CL & Quinn, JP 2002, Electron capture dissociation: Optimization, mechanism, and applications. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 107-108, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Marshall AG, Chalmers MJ, Cooper HJ, Emmett M, Håkansson K, Hendrickson CL et al. Electron capture dissociation: Optimization, mechanism, and applications. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 107-108
Marshall, A. G. ; Chalmers, M. J. ; Cooper, H. J. ; Emmett, Mark ; Håkansson, K. ; Hendrickson, C. L. ; Hudgins, R. R. ; Nilsson, C. L. ; Quinn, J. P. / Electron capture dissociation : Optimization, mechanism, and applications. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 107-108
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