Electron capture dissociation: Optimization, mechanism, and applications

A. G. Marshall; M. J. Chalmers; H. J. Cooper; Mark Emmett; K. Håkansson; C. L. Hendrickson; R. R. Hudgins; C. L. Nilsson; J. P. Quinn

Electron capture dissociation: Optimization, mechanism, and applications

A. G. Marshall
, M. J. Chalmers
, H. J. Cooper
, Mark Emmett
, K. Håkansson
, C. L. Hendrickson
, R. R. Hudgins
, C. L. Nilsson
, J. P. Quinn

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The application of electron capture dissociation (ECD) as an analytical method for determining peptide and protein primary amino acid sequences as well as post-translational modification sites is discussed. A 7.0 and 9.4 T homebuilt Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers were used to conduct ECD. Multiply-charged cations were generated by microelectrospray ionization and to permit infrared multiphoton dissociation (IRMPD) an ECD dispenser electrode was positioned off-axis. The results show amino acid side-chain cleavages for arginine, histidine, asparagine/glutamine, methionine and lysine residues.

Original language	English
Title of host publication	Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	107-108
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Marshall, AG, Chalmers, MJ, Cooper, HJ, Emmett, M, Håkansson, K, Hendrickson, CL, Hudgins, RR, Nilsson, CL & Quinn, JP 2002, Electron capture dissociation: Optimization, mechanism, and applications. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 107-108, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

@inproceedings{a64cfb021b354d4fb355475c43836bc6,

title = "Electron capture dissociation: Optimization, mechanism, and applications",

abstract = "The application of electron capture dissociation (ECD) as an analytical method for determining peptide and protein primary amino acid sequences as well as post-translational modification sites is discussed. A 7.0 and 9.4 T homebuilt Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers were used to conduct ECD. Multiply-charged cations were generated by microelectrospray ionization and to permit infrared multiphoton dissociation (IRMPD) an ECD dispenser electrode was positioned off-axis. The results show amino acid side-chain cleavages for arginine, histidine, asparagine/glutamine, methionine and lysine residues.",

author = "Marshall, \{A. G.\} and Chalmers, \{M. J.\} and Cooper, \{H. J.\} and Mark Emmett and K. H{\aa}kansson and Hendrickson, \{C. L.\} and Hudgins, \{R. R.\} and Nilsson, \{C. L.\} and Quinn, \{J. P.\}",

year = "2002",

language = "English",

pages = "107--108",

booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - GEN

T1 - Electron capture dissociation

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

AU - Marshall, A. G.

AU - Chalmers, M. J.

AU - Cooper, H. J.

AU - Emmett, Mark

AU - Håkansson, K.

AU - Hendrickson, C. L.

AU - Hudgins, R. R.

AU - Nilsson, C. L.

AU - Quinn, J. P.

PY - 2002

Y1 - 2002

N2 - The application of electron capture dissociation (ECD) as an analytical method for determining peptide and protein primary amino acid sequences as well as post-translational modification sites is discussed. A 7.0 and 9.4 T homebuilt Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers were used to conduct ECD. Multiply-charged cations were generated by microelectrospray ionization and to permit infrared multiphoton dissociation (IRMPD) an ECD dispenser electrode was positioned off-axis. The results show amino acid side-chain cleavages for arginine, histidine, asparagine/glutamine, methionine and lysine residues.

AB - The application of electron capture dissociation (ECD) as an analytical method for determining peptide and protein primary amino acid sequences as well as post-translational modification sites is discussed. A 7.0 and 9.4 T homebuilt Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers were used to conduct ECD. Multiply-charged cations were generated by microelectrospray ionization and to permit infrared multiphoton dissociation (IRMPD) an ECD dispenser electrode was positioned off-axis. The results show amino acid side-chain cleavages for arginine, histidine, asparagine/glutamine, methionine and lysine residues.

UR - https://www.scopus.com/pages/publications/2442647781

UR - https://www.scopus.com/pages/publications/2442647781#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:2442647781

SP - 107

EP - 108

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Electron capture dissociation: Optimization, mechanism, and applications

Abstract

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ASJC Scopus subject areas

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