Electron cryomicroscopy and angular reconstitution used to visualize the skeletal muscle calcium release channel

Irina I. Serysheva, Elena V. Orlova, Wah Chiu, Michael B. Sherman, Susan L. Hamilton, Marin Van Heel

Research output: Contribution to journalArticle

163 Scopus citations

Abstract

We exploit the random orientations of ice-embedded molecules imaged in an electron cryomicroscope to determine the three-dimensional structure of the Ca2+-release channel from the sarcoplasmic reticulum (SR) in its closed state, without tilting the specimen holder. Our new reconstruction approach includes an exhaustive search of all different characteristic projection images in the micrographs and the assignment of Euler angle orientations to these views. The 30 Å map implied reveals a structure in which the transmembrane region exhibits no apparent opening on the SR lumen side. The extended cytoplasmic region has a hollow appearance and consists, in each monomer, of a clampshaped and a handle-shaped domain.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalNature Structural Biology
Volume2
Issue number1
DOIs
StatePublished - Jan 1995
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics

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