Electron microscopic analysis of infection patterns for Venezuelan equine encephalomyelitis virus in the vector mosquito, Culex (Melanoconion) taeniopus

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Abstract

The infectious process for Venezuelan equine encephalomyelitis virus in an enzootic vector mosquito, Culex (Melanoconion) taeniopus, was examined by transmission electron microscopy. Following large dose oral infection, virus was seen in the abdominal fat body and epidermis within 1 hr of engorgement. Replicated virus appeared to leave the mesenteron 3-4 hr post-infection. Dissemination to other organs occurred between days 1 and 2 of extrinsic incubation, when the hindgut and abdominal nerve ganglia were found to be infected. Virus reached the thoracic nerve ganglia, brain and salivary glands by 4 days post-infection, and flight muscles contained virus by day 6. Virus was never detected in the malpighian tubules or ovaries of infected mosquitoes within 21 days of extrinsic incubation. These results suggest that virus particles penetrate the mesenteron and accumulate in the abdominal fat body prior to replication within the vector. This pattern differs from that reported for other arbovirus-vector pairs.

Original languageEnglish (US)
Pages (from-to)624-631
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume35
Issue number3
StatePublished - 1986
Externally publishedYes

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Venezuelan Equine Encephalitis Viruses
Culex
Electrons
Viruses
Abdominal Fat
Fat Body
Infection
Ganglia
Thoracic Nerves
Malpighian Tubules
Arboviruses
Virus Diseases
Salivary Glands
Culicidae
Transmission Electron Microscopy
Epidermis
Virion
Ovary
Muscles
Mosquito Vectors

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

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abstract = "The infectious process for Venezuelan equine encephalomyelitis virus in an enzootic vector mosquito, Culex (Melanoconion) taeniopus, was examined by transmission electron microscopy. Following large dose oral infection, virus was seen in the abdominal fat body and epidermis within 1 hr of engorgement. Replicated virus appeared to leave the mesenteron 3-4 hr post-infection. Dissemination to other organs occurred between days 1 and 2 of extrinsic incubation, when the hindgut and abdominal nerve ganglia were found to be infected. Virus reached the thoracic nerve ganglia, brain and salivary glands by 4 days post-infection, and flight muscles contained virus by day 6. Virus was never detected in the malpighian tubules or ovaries of infected mosquitoes within 21 days of extrinsic incubation. These results suggest that virus particles penetrate the mesenteron and accumulate in the abdominal fat body prior to replication within the vector. This pattern differs from that reported for other arbovirus-vector pairs.",
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