Electron transport chain dysfunction by H2O2 is linked to increased reactive oxygen species production and iron mobilization by lipoperoxidation: Studies using Saccharomyces cerevisiae mitochondria

Christian Cortés-Rojo, Mirella Estrada-Villagómez, Elizabeth Calderón-Cortés, Mónica Clemente-Guerrero, Ricardo Mejía-Zepeda, Istvan Boldogh, Alfredo Saavedra-Molina

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The mitochondrial electron transport chain (ETC) contains thiol groups (-SH) which are reversibly oxidized to modulate ETC function during H 2O2 overproduction. Since deleterious effects of H 2O2 are not limited to -SH oxidation, due to the formation of other H2O2-derived species, some processes like lipoperoxidation could enhance the effects of H2O2 over ETC enzymes, disrupt their modulation by -SH oxidation and increase superoxide production. To verify this hypothesis, we tested the effects of H 2O2 on ETC activities, superoxide production and iron mobilization in mitochondria from lipoperoxidation-resistant native yeast and lipoperoxidation-sensitized yeast. Only complex III activity from lipoperoxidation-sensitive mitochondria exhibited a higher susceptibility to H2O2 and increased superoxide production. The recovery of ETC activity by the thiol reductanct β-mercaptoethanol (BME) was also altered at complex III, and a role was attributed to lipoperoxidation, the latter being also responsible for iron release. A hypothetical model linking lipoperoxidation, increased complex III damage, superoxide production and iron release is given.

Original languageEnglish (US)
Pages (from-to)135-147
Number of pages13
JournalJournal of Bioenergetics and Biomembranes
Volume43
Issue number2
DOIs
StatePublished - Apr 2011

Keywords

  • Linolenic acid
  • Lipid peroxidation
  • Oxidative stress
  • Respiratory chain
  • Thiol oxidation
  • Yeast
  • β-mercaptoethanol

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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