Electron transport chain of Saccharomyces cerevisiae mitochondria is inhibited by H2O2 at succinate-cytochrome c oxidoreductase level without lipid peroxidation involvement

Christian Cortés-Rojo, Elizabeth Calderó;n-Cortés, Mónica Clemente-Guerrero, Salvador Manzo-Ávalos, Salvador Uribe, Istvan Boldogh, Alfredo Saavedra-Molina

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The deleterious effects of H2O2 on the electron transport chain of yeast mitochondria and on mitochondrial lipid peroxidation were evaluated. Exposure to H2O2 resulted in inhibition of the oxygen consumption in the uncoupled and phosphorylating states to 69% and 65%, respectively. The effect of H2O2 on the respiratory rate was associated with an inhibition of succinate-ubiquinone and succinate-DCIP oxidoreductase activities. Inhibitory effect of H2O2 on respiratory complexes was almost completely recovered by β-mercaptoethanol treatment. H2O2 treatment resulted in full resistance to QO site inhibitor myxothiazol and thus it is suggested that the quinol oxidase site (QO) of complex III is the target for H2O2. H2O2 did not modify basal levels of lipid peroxidation in yeast mitochondria. However, H2O2 addition to rat brain and liver mitochondria induced an increase in lipid peroxidation. These results are discussed in terms of the known physiological differences between mammalian and yeast mitochondria.

Original languageEnglish (US)
Pages (from-to)1212-1223
Number of pages12
JournalFree Radical Research
Volume41
Issue number11
DOIs
StatePublished - Nov 1 2007

Keywords

  • HO
  • Mitochondria
  • Oxygen consumption
  • Peroxidation
  • Respiratory chain
  • Yeast

ASJC Scopus subject areas

  • Biochemistry

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