Electrophysiological and biochemical characteristics of Gymnodinium breve toxins

J. L. Parmentier; T. Narahashi; W. A. Wilson; N. M. Trieff; V. M.Sadagopa Ramanujam; M. Risk; S. M. Ray

doi:10.1016/0041-0101(78)90084-3

Electrophysiological and biochemical characteristics of Gymnodinium breve toxins

J. L. Parmentier, T. Narahashi, W. A. Wilson, N. M. Trieff, V. M.Sadagopa Ramanujam, M. Risk, S. M. Ray

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Five toxins (T₁-T₅) have been isolated from a crude acidified ether extract of unialgal cultures of the dinoflagellate Gymnodinium breve Davis using techniques of column, liquid and thin layer chromatography. Electrophysiological studies using the spontaneously active ventral nerve cord preparation of the crayfish showed that two of the fractions, T₂ and T₄ caused excitation to more than 250% of control levels at a concentration of 0·05 ng/ml. A blockage of firing occurred at higher concentrations. Fractions T₁, T₃ and T₅ produced gradual excitation only at the higher concentrations. T₂ and T₄ at a concentration of 250 ng/ml also produced excitation and irregular firing in central neurons from Aplysia californica. Studies on the effect of purified toxins on total choline incorporation in C₆1A rat glia clone cultures indicate that toxins T₂-T₅ increase lipid vs nonlipid choline metabolites, presumably by base exchange stimulation. Only T₂ stimulated choline uptake in this system.

Original language	English (US)
Pages (from-to)	235-244
Number of pages	10
Journal	Toxicon
Volume	16
Issue number	3
DOIs	https://doi.org/10.1016/0041-0101(78)90084-3
State	Published - 1978
Externally published	Yes

ASJC Scopus subject areas

Toxicology

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@article{6931fb15d9c34e12942b25125b13cbb1,

title = "Electrophysiological and biochemical characteristics of Gymnodinium breve toxins",

abstract = "Five toxins (T1-T5) have been isolated from a crude acidified ether extract of unialgal cultures of the dinoflagellate Gymnodinium breve Davis using techniques of column, liquid and thin layer chromatography. Electrophysiological studies using the spontaneously active ventral nerve cord preparation of the crayfish showed that two of the fractions, T2 and T4 caused excitation to more than 250% of control levels at a concentration of 0·05 ng/ml. A blockage of firing occurred at higher concentrations. Fractions T1, T3 and T5 produced gradual excitation only at the higher concentrations. T2 and T4 at a concentration of 250 ng/ml also produced excitation and irregular firing in central neurons from Aplysia californica. Studies on the effect of purified toxins on total choline incorporation in C61A rat glia clone cultures indicate that toxins T2-T5 increase lipid vs nonlipid choline metabolites, presumably by base exchange stimulation. Only T2 stimulated choline uptake in this system.",

author = "Parmentier, {J. L.} and T. Narahashi and Wilson, {W. A.} and Trieff, {N. M.} and Ramanujam, {V. M.Sadagopa} and M. Risk and Ray, {S. M.}",

note = "Funding Information: Acknowledgements-This work was supported by a grant from tho Robert A. Wekh Foundation, Houston, Texas (H-416 to Dr . TRIEFF), the Fund for Organized Research, Texas A & M University (to Dr. RAY), and a grant from tho National Institutes of Health (NS 10823 to Dr. NAeAFtASFn).",

year = "1978",

doi = "10.1016/0041-0101(78)90084-3",

language = "English (US)",

volume = "16",

pages = "235--244",

journal = "Toxicon",

issn = "0041-0101",

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number = "3",

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T1 - Electrophysiological and biochemical characteristics of Gymnodinium breve toxins

AU - Parmentier, J. L.

AU - Narahashi, T.

AU - Wilson, W. A.

AU - Trieff, N. M.

AU - Ramanujam, V. M.Sadagopa

AU - Risk, M.

AU - Ray, S. M.

N1 - Funding Information: Acknowledgements-This work was supported by a grant from tho Robert A. Wekh Foundation, Houston, Texas (H-416 to Dr . TRIEFF), the Fund for Organized Research, Texas A & M University (to Dr. RAY), and a grant from tho National Institutes of Health (NS 10823 to Dr. NAeAFtASFn).

PY - 1978

Y1 - 1978

N2 - Five toxins (T1-T5) have been isolated from a crude acidified ether extract of unialgal cultures of the dinoflagellate Gymnodinium breve Davis using techniques of column, liquid and thin layer chromatography. Electrophysiological studies using the spontaneously active ventral nerve cord preparation of the crayfish showed that two of the fractions, T2 and T4 caused excitation to more than 250% of control levels at a concentration of 0·05 ng/ml. A blockage of firing occurred at higher concentrations. Fractions T1, T3 and T5 produced gradual excitation only at the higher concentrations. T2 and T4 at a concentration of 250 ng/ml also produced excitation and irregular firing in central neurons from Aplysia californica. Studies on the effect of purified toxins on total choline incorporation in C61A rat glia clone cultures indicate that toxins T2-T5 increase lipid vs nonlipid choline metabolites, presumably by base exchange stimulation. Only T2 stimulated choline uptake in this system.

AB - Five toxins (T1-T5) have been isolated from a crude acidified ether extract of unialgal cultures of the dinoflagellate Gymnodinium breve Davis using techniques of column, liquid and thin layer chromatography. Electrophysiological studies using the spontaneously active ventral nerve cord preparation of the crayfish showed that two of the fractions, T2 and T4 caused excitation to more than 250% of control levels at a concentration of 0·05 ng/ml. A blockage of firing occurred at higher concentrations. Fractions T1, T3 and T5 produced gradual excitation only at the higher concentrations. T2 and T4 at a concentration of 250 ng/ml also produced excitation and irregular firing in central neurons from Aplysia californica. Studies on the effect of purified toxins on total choline incorporation in C61A rat glia clone cultures indicate that toxins T2-T5 increase lipid vs nonlipid choline metabolites, presumably by base exchange stimulation. Only T2 stimulated choline uptake in this system.

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Electrophysiological and biochemical characteristics of Gymnodinium breve toxins

Abstract

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