Electrophysiological and biochemical characteristics of Gymnodinium breve toxins

J. L. Parmentier, T. Narahashi, W. A. Wilson, N. M. Trieff, V-M Ramanujam, M. Risk, S. M. Ray

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Five toxins (T1-T5) have been isolated from a crude acidified ether extract of unialgal cultures of the dinoflagellate Gymnodinium breve Davis using techniques of column, liquid and thin layer chromatography. Electrophysiological studies using the spontaneously active ventral nerve cord preparation of the crayfish showed that two of the fractions, T2 and T4 caused excitation to more than 250% of control levels at a concentration of 0·05 ng/ml. A blockage of firing occurred at higher concentrations. Fractions T1, T3 and T5 produced gradual excitation only at the higher concentrations. T2 and T4 at a concentration of 250 ng/ml also produced excitation and irregular firing in central neurons from Aplysia californica. Studies on the effect of purified toxins on total choline incorporation in C61A rat glia clone cultures indicate that toxins T2-T5 increase lipid vs nonlipid choline metabolites, presumably by base exchange stimulation. Only T2 stimulated choline uptake in this system.

Original languageEnglish (US)
Pages (from-to)235-244
Number of pages10
JournalToxicon
Volume16
Issue number3
DOIs
StatePublished - 1978

Fingerprint

Choline
Dinoflagellida
T-2 Toxin
Aplysia
Thin layer chromatography
Astacoidea
Level control
Thin Layer Chromatography
Metabolites
Neuroglia
Ether
Neurons
Rats
Clone Cells
Lipids
Liquids
Gymnodinium breve toxin

ASJC Scopus subject areas

  • Toxicology

Cite this

Parmentier, J. L., Narahashi, T., Wilson, W. A., Trieff, N. M., Ramanujam, V-M., Risk, M., & Ray, S. M. (1978). Electrophysiological and biochemical characteristics of Gymnodinium breve toxins. Toxicon, 16(3), 235-244. https://doi.org/10.1016/0041-0101(78)90084-3

Electrophysiological and biochemical characteristics of Gymnodinium breve toxins. / Parmentier, J. L.; Narahashi, T.; Wilson, W. A.; Trieff, N. M.; Ramanujam, V-M; Risk, M.; Ray, S. M.

In: Toxicon, Vol. 16, No. 3, 1978, p. 235-244.

Research output: Contribution to journalArticle

Parmentier, JL, Narahashi, T, Wilson, WA, Trieff, NM, Ramanujam, V-M, Risk, M & Ray, SM 1978, 'Electrophysiological and biochemical characteristics of Gymnodinium breve toxins', Toxicon, vol. 16, no. 3, pp. 235-244. https://doi.org/10.1016/0041-0101(78)90084-3
Parmentier, J. L. ; Narahashi, T. ; Wilson, W. A. ; Trieff, N. M. ; Ramanujam, V-M ; Risk, M. ; Ray, S. M. / Electrophysiological and biochemical characteristics of Gymnodinium breve toxins. In: Toxicon. 1978 ; Vol. 16, No. 3. pp. 235-244.
@article{6931fb15d9c34e12942b25125b13cbb1,
title = "Electrophysiological and biochemical characteristics of Gymnodinium breve toxins",
abstract = "Five toxins (T1-T5) have been isolated from a crude acidified ether extract of unialgal cultures of the dinoflagellate Gymnodinium breve Davis using techniques of column, liquid and thin layer chromatography. Electrophysiological studies using the spontaneously active ventral nerve cord preparation of the crayfish showed that two of the fractions, T2 and T4 caused excitation to more than 250{\%} of control levels at a concentration of 0·05 ng/ml. A blockage of firing occurred at higher concentrations. Fractions T1, T3 and T5 produced gradual excitation only at the higher concentrations. T2 and T4 at a concentration of 250 ng/ml also produced excitation and irregular firing in central neurons from Aplysia californica. Studies on the effect of purified toxins on total choline incorporation in C61A rat glia clone cultures indicate that toxins T2-T5 increase lipid vs nonlipid choline metabolites, presumably by base exchange stimulation. Only T2 stimulated choline uptake in this system.",
author = "Parmentier, {J. L.} and T. Narahashi and Wilson, {W. A.} and Trieff, {N. M.} and V-M Ramanujam and M. Risk and Ray, {S. M.}",
year = "1978",
doi = "10.1016/0041-0101(78)90084-3",
language = "English (US)",
volume = "16",
pages = "235--244",
journal = "Toxicon",
issn = "0041-0101",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Electrophysiological and biochemical characteristics of Gymnodinium breve toxins

AU - Parmentier, J. L.

AU - Narahashi, T.

AU - Wilson, W. A.

AU - Trieff, N. M.

AU - Ramanujam, V-M

AU - Risk, M.

AU - Ray, S. M.

PY - 1978

Y1 - 1978

N2 - Five toxins (T1-T5) have been isolated from a crude acidified ether extract of unialgal cultures of the dinoflagellate Gymnodinium breve Davis using techniques of column, liquid and thin layer chromatography. Electrophysiological studies using the spontaneously active ventral nerve cord preparation of the crayfish showed that two of the fractions, T2 and T4 caused excitation to more than 250% of control levels at a concentration of 0·05 ng/ml. A blockage of firing occurred at higher concentrations. Fractions T1, T3 and T5 produced gradual excitation only at the higher concentrations. T2 and T4 at a concentration of 250 ng/ml also produced excitation and irregular firing in central neurons from Aplysia californica. Studies on the effect of purified toxins on total choline incorporation in C61A rat glia clone cultures indicate that toxins T2-T5 increase lipid vs nonlipid choline metabolites, presumably by base exchange stimulation. Only T2 stimulated choline uptake in this system.

AB - Five toxins (T1-T5) have been isolated from a crude acidified ether extract of unialgal cultures of the dinoflagellate Gymnodinium breve Davis using techniques of column, liquid and thin layer chromatography. Electrophysiological studies using the spontaneously active ventral nerve cord preparation of the crayfish showed that two of the fractions, T2 and T4 caused excitation to more than 250% of control levels at a concentration of 0·05 ng/ml. A blockage of firing occurred at higher concentrations. Fractions T1, T3 and T5 produced gradual excitation only at the higher concentrations. T2 and T4 at a concentration of 250 ng/ml also produced excitation and irregular firing in central neurons from Aplysia californica. Studies on the effect of purified toxins on total choline incorporation in C61A rat glia clone cultures indicate that toxins T2-T5 increase lipid vs nonlipid choline metabolites, presumably by base exchange stimulation. Only T2 stimulated choline uptake in this system.

UR - http://www.scopus.com/inward/record.url?scp=0017857390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017857390&partnerID=8YFLogxK

U2 - 10.1016/0041-0101(78)90084-3

DO - 10.1016/0041-0101(78)90084-3

M3 - Article

VL - 16

SP - 235

EP - 244

JO - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 3

ER -