Electrophysiological and morphological properties of neurons acutely isolated from the rostral gustatory zone of the rat nucleus of the solitary tract

Junhui Du, Robert M. Bradley

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The biophysical and morphological characteristics of acutely isolated neurons from the rostral nucleus of the solitary tract (rNST) were investigated under current clamp conditions and compared with the results obtained from neurons recorded in brain slices. The passive membrane properties of the isolated neurons were similar to rNST neurons in brain slices and the neurons maintained their morphological characteristics although their dendritic tree was truncated. The isolated neurons also retained their characteristic repetitive firing properties. In addition we also noted developmental changes in the intrinsic membrane properties of the isolated neurons, such as a shortening in action potential duration, decrease in membrane time constant and input resistance, that occurred when these parameters were compared in neurons isolated from young (5-10 days) and older animals. These enzymatically dispersed neurons therefore retained both the membrane properties and morphology observed in the intact brainstem and in vitro brain slice preparation. The use of this isolated neuron preparation provides a basis for further study of rNST neurobiology.

    Original languageEnglish (US)
    Pages (from-to)729-737
    Number of pages9
    JournalChemical Senses
    Volume21
    Issue number6
    DOIs
    StatePublished - 1996

    Fingerprint

    Solitary Nucleus
    neurons
    Neurons
    rats
    Membranes
    brain
    Brain
    neurophysiology
    Neurobiology
    brain stem
    action potentials
    shortenings
    Action Potentials
    Brain Stem

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences (miscellaneous)
    • Food Science
    • Neuroscience(all)
    • Physiology
    • Physiology (medical)
    • Behavioral Neuroscience

    Cite this

    Electrophysiological and morphological properties of neurons acutely isolated from the rostral gustatory zone of the rat nucleus of the solitary tract. / Du, Junhui; Bradley, Robert M.

    In: Chemical Senses, Vol. 21, No. 6, 1996, p. 729-737.

    Research output: Contribution to journalArticle

    @article{a2847333e5bf4873a453a5fd90b18b12,
    title = "Electrophysiological and morphological properties of neurons acutely isolated from the rostral gustatory zone of the rat nucleus of the solitary tract",
    abstract = "The biophysical and morphological characteristics of acutely isolated neurons from the rostral nucleus of the solitary tract (rNST) were investigated under current clamp conditions and compared with the results obtained from neurons recorded in brain slices. The passive membrane properties of the isolated neurons were similar to rNST neurons in brain slices and the neurons maintained their morphological characteristics although their dendritic tree was truncated. The isolated neurons also retained their characteristic repetitive firing properties. In addition we also noted developmental changes in the intrinsic membrane properties of the isolated neurons, such as a shortening in action potential duration, decrease in membrane time constant and input resistance, that occurred when these parameters were compared in neurons isolated from young (5-10 days) and older animals. These enzymatically dispersed neurons therefore retained both the membrane properties and morphology observed in the intact brainstem and in vitro brain slice preparation. The use of this isolated neuron preparation provides a basis for further study of rNST neurobiology.",
    author = "Junhui Du and Bradley, {Robert M.}",
    year = "1996",
    doi = "10.1093/chemse/21.6.729",
    language = "English (US)",
    volume = "21",
    pages = "729--737",
    journal = "Chemical Senses",
    issn = "0379-864X",
    publisher = "Oxford University Press",
    number = "6",

    }

    TY - JOUR

    T1 - Electrophysiological and morphological properties of neurons acutely isolated from the rostral gustatory zone of the rat nucleus of the solitary tract

    AU - Du, Junhui

    AU - Bradley, Robert M.

    PY - 1996

    Y1 - 1996

    N2 - The biophysical and morphological characteristics of acutely isolated neurons from the rostral nucleus of the solitary tract (rNST) were investigated under current clamp conditions and compared with the results obtained from neurons recorded in brain slices. The passive membrane properties of the isolated neurons were similar to rNST neurons in brain slices and the neurons maintained their morphological characteristics although their dendritic tree was truncated. The isolated neurons also retained their characteristic repetitive firing properties. In addition we also noted developmental changes in the intrinsic membrane properties of the isolated neurons, such as a shortening in action potential duration, decrease in membrane time constant and input resistance, that occurred when these parameters were compared in neurons isolated from young (5-10 days) and older animals. These enzymatically dispersed neurons therefore retained both the membrane properties and morphology observed in the intact brainstem and in vitro brain slice preparation. The use of this isolated neuron preparation provides a basis for further study of rNST neurobiology.

    AB - The biophysical and morphological characteristics of acutely isolated neurons from the rostral nucleus of the solitary tract (rNST) were investigated under current clamp conditions and compared with the results obtained from neurons recorded in brain slices. The passive membrane properties of the isolated neurons were similar to rNST neurons in brain slices and the neurons maintained their morphological characteristics although their dendritic tree was truncated. The isolated neurons also retained their characteristic repetitive firing properties. In addition we also noted developmental changes in the intrinsic membrane properties of the isolated neurons, such as a shortening in action potential duration, decrease in membrane time constant and input resistance, that occurred when these parameters were compared in neurons isolated from young (5-10 days) and older animals. These enzymatically dispersed neurons therefore retained both the membrane properties and morphology observed in the intact brainstem and in vitro brain slice preparation. The use of this isolated neuron preparation provides a basis for further study of rNST neurobiology.

    UR - http://www.scopus.com/inward/record.url?scp=0030445301&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0030445301&partnerID=8YFLogxK

    U2 - 10.1093/chemse/21.6.729

    DO - 10.1093/chemse/21.6.729

    M3 - Article

    C2 - 8985601

    AN - SCOPUS:0030445301

    VL - 21

    SP - 729

    EP - 737

    JO - Chemical Senses

    JF - Chemical Senses

    SN - 0379-864X

    IS - 6

    ER -