Electrophysiological evaluation of extracellular spermine and alkaline pH on synaptic human GABAA receptors

Agenor Limon-Ruiz, E. Delbruck, A. Yassine, D. Pandya, R. M. Myers, J. D. Barchas, F. Lee, Schatzberg, S. J. Watson, H. Akil, W. E. Bunney, M. P. Vawter, A. Sequeira

Research output: Contribution to journalArticle

Abstract

Polyamines have fundamental roles in brain homeostasis as key modulators of cellular excitability. Several studies have suggested alterations in polyamine metabolism in stress related disorders, suicide, depression, and neurodegeneration, making the pharmacological modulation of polyamines a highly appealing therapeutic strategy. Polyamines are small aliphatic molecules that can modulate cationic channels involved in neuronal excitability. Previous indirect evidence has suggested that polyamines can modulate anionic GABAA receptors (GABAARs), which mediate inhibitory signaling and provide a direct route to reduce hyperexcitability. Here, we attempted to characterize the effect that spermine, the polyamine with the strongest reported effect on GABAARs, has on human postmortem native GABAARs. We microtransplanted human synaptic membranes from the dorsolateral prefrontal cortex of four cases with no history of mental or neurological disorders, and directly recorded spermine effects on ionic GABAARs responses on microtransplanted oocytes. We show that in human synapses, inhibition of GABAARs by spermine was better explained by alkalization of the extracellular solution. Additionally, spermine had no effect on the potentiation of GABA-currents by diazepam, indicating that even if diazepam binding is enhanced by spermine, it does not translate to changes in functional activity. Our results clearly demonstrate that while extracellular spermine does not have direct effects on human native synaptic GABAARs, spermine-mediated shifts of pH inhibit GABAARs. Potential spermine-mediated increase of pH in synapses in vivo may therefore participate in increased neuronal activity observed during physiological and pathological states, and during metabolic alterations that increase the release of spermine to the extracellular milieu.

Original languageEnglish (US)
Article number218
JournalTranslational psychiatry
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

Spermine
GABA-A Receptors
Polyamines
Diazepam
Synapses
Synaptic Membranes
Neurotransmitter Receptor
Prefrontal Cortex
Nervous System Diseases
Mental Disorders
gamma-Aminobutyric Acid
Suicide
Oocytes
Homeostasis
Pharmacology
Depression
Brain

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience
  • Biological Psychiatry

Cite this

Electrophysiological evaluation of extracellular spermine and alkaline pH on synaptic human GABAA receptors. / Limon-Ruiz, Agenor; Delbruck, E.; Yassine, A.; Pandya, D.; Myers, R. M.; Barchas, J. D.; Lee, F.; Schatzberg; Watson, S. J.; Akil, H.; Bunney, W. E.; Vawter, M. P.; Sequeira, A.

In: Translational psychiatry, Vol. 9, No. 1, 218, 01.12.2019.

Research output: Contribution to journalArticle

Limon-Ruiz, A, Delbruck, E, Yassine, A, Pandya, D, Myers, RM, Barchas, JD, Lee, F, Schatzberg, Watson, SJ, Akil, H, Bunney, WE, Vawter, MP & Sequeira, A 2019, 'Electrophysiological evaluation of extracellular spermine and alkaline pH on synaptic human GABAA receptors', Translational psychiatry, vol. 9, no. 1, 218. https://doi.org/10.1038/s41398-019-0551-1
Limon-Ruiz, Agenor ; Delbruck, E. ; Yassine, A. ; Pandya, D. ; Myers, R. M. ; Barchas, J. D. ; Lee, F. ; Schatzberg ; Watson, S. J. ; Akil, H. ; Bunney, W. E. ; Vawter, M. P. ; Sequeira, A. / Electrophysiological evaluation of extracellular spermine and alkaline pH on synaptic human GABAA receptors. In: Translational psychiatry. 2019 ; Vol. 9, No. 1.
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