Elevated frequencies of hprt mutant lymphocytes in cigarette-smoking mothers and their newborns

Marinel M. Ammenheuser, Abbey Berenson, Norma J. Stiglich, Elbert B. Whorton, Jonathan B. Ward

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Maternal cigarette smoking during pregnancy has been associated with increased perinatal mortality and low birth weight. Several epidemiological studies have demonstrated an association between smoking during pregnancy and an elevated risk of hematopoietic cancer in the child, but other studies have failed to confirm this association. We have used an assay for somatic cell mutation to evaluate the in utero effects of exposure to maternal cigarette smoking. Cord blood samples were obtained from 10 newborns whose mothers smoked cigarettes during pregnancy and 10 newborns of non-smoking mothers. Blood samples were also obtained from 5 of the smoking and 5 of the non-smoking mothers. Smoking status was confirmed in all samples by testing the blood plasma for cotinine. The frequency of lymphocytes containing mutations at the hypoxanthine phosphoribosyltransferase (hprt) locus was determined with an autoradiographic assay using cells that had been cryopreserved. The mothers who were smokers had a mean frequency (±SE) of 3.08 (±0.55) variant (mutant) cells per 106 evaluatable lymphocytes. The frequency (Vf) in non-smokers was 1.07 (±0.17) × 10-6. The Vf of newborns of smokers was 2.17 ±0.24 × 10-6, and new borns of non-smokers had a Vfof 0.77 (±0.13) × 10-6. In both mothers and newborns the difference in Vf between smokers and non-smokers was statistically significant (p < 0.05). Maternal and newborn Vfs were significantly correlated (r = 0.88; p < 0.004), and there was a positive association (r = 0.86; p < 0.001) between the reported number of cigarettes smoked per day and the Vfs. This study provides further evidence that maternal smoking may be hazardous to the future health of children exposed in utero to mutagenic agents in cigarette smoke.

Original languageEnglish (US)
Pages (from-to)285-294
Number of pages10
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume304
Issue number2
DOIs
StatePublished - Jan 16 1994

Fingerprint

Hypoxanthine Phosphoribosyltransferase
Smoking
Mothers
Newborn Infant
Lymphocytes
Tobacco Products
Pregnancy
Maternal Exposure
Cotinine
Mutation
Perinatal Mortality
Low Birth Weight Infant
Fetal Blood
Smoke
Epidemiologic Studies

Keywords

  • Cotinine
  • Genetic monitoring
  • Hprt mutation
  • Humans
  • Lymphocytes
  • Pregnancy
  • Smoking
  • Tobacco

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis

Cite this

Elevated frequencies of hprt mutant lymphocytes in cigarette-smoking mothers and their newborns. / Ammenheuser, Marinel M.; Berenson, Abbey; Stiglich, Norma J.; Whorton, Elbert B.; Ward, Jonathan B.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 304, No. 2, 16.01.1994, p. 285-294.

Research output: Contribution to journalArticle

Ammenheuser, Marinel M. ; Berenson, Abbey ; Stiglich, Norma J. ; Whorton, Elbert B. ; Ward, Jonathan B. / Elevated frequencies of hprt mutant lymphocytes in cigarette-smoking mothers and their newborns. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 1994 ; Vol. 304, No. 2. pp. 285-294.
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AU - Ward, Jonathan B.

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AB - Maternal cigarette smoking during pregnancy has been associated with increased perinatal mortality and low birth weight. Several epidemiological studies have demonstrated an association between smoking during pregnancy and an elevated risk of hematopoietic cancer in the child, but other studies have failed to confirm this association. We have used an assay for somatic cell mutation to evaluate the in utero effects of exposure to maternal cigarette smoking. Cord blood samples were obtained from 10 newborns whose mothers smoked cigarettes during pregnancy and 10 newborns of non-smoking mothers. Blood samples were also obtained from 5 of the smoking and 5 of the non-smoking mothers. Smoking status was confirmed in all samples by testing the blood plasma for cotinine. The frequency of lymphocytes containing mutations at the hypoxanthine phosphoribosyltransferase (hprt) locus was determined with an autoradiographic assay using cells that had been cryopreserved. The mothers who were smokers had a mean frequency (±SE) of 3.08 (±0.55) variant (mutant) cells per 106 evaluatable lymphocytes. The frequency (Vf) in non-smokers was 1.07 (±0.17) × 10-6. The Vf of newborns of smokers was 2.17 ±0.24 × 10-6, and new borns of non-smokers had a Vfof 0.77 (±0.13) × 10-6. In both mothers and newborns the difference in Vf between smokers and non-smokers was statistically significant (p < 0.05). Maternal and newborn Vfs were significantly correlated (r = 0.88; p < 0.004), and there was a positive association (r = 0.86; p < 0.001) between the reported number of cigarettes smoked per day and the Vfs. This study provides further evidence that maternal smoking may be hazardous to the future health of children exposed in utero to mutagenic agents in cigarette smoke.

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