Elevated interleukin-6 expression in keloid fibroblasts

Hui Xue, Robert L. McCauley, Wenru Zhang

    Research output: Contribution to journalArticle

    64 Citations (Scopus)

    Abstract

    Keloids are characterized by a net accumulation of collagen. To date, the role of growth factors and various cytokines in the pathogenesis of these lesions has not been fully characterized. Interleukin-6 (IL-6) is an important immunoregulatory cytokine that has been implicated in a number of fibrotic autoimmune diseases such as scleroderma, interstitial nephritis, and pulmonary interstitial fibrosis. However, the role of IL-6 in the development of keloids has yet to be defined. This study demonstrates increased expression of the IL-6 gene in fibroblasts isolated from patients with keloids when compared with control fibroblasts using the ribonuclease protection assay. Subsequent detection of increased levels of IL-6 secretion by keloid fibroblasts is also demonstrated under unstimulated and stimulated conditions using serum and interferon γ (IFN-γ) (unstimulated: 0.3694 + 0.2499 pg/cell vs 0.0662 + 0.0786 pg/cell, P = 0.0137; serum: 1.066 + 0.513 pg/cell vs 0.233 + 0.231 pg/cell, P = 0.0027; serum and IFN-γ 1.286 + 0.395 pg/cell vs 0.244 + 0.199 pg/cell, P < 0.0001). These results suggest that IL- 6 may play a significant role in the pathogenesis of keloids. (C) 2000 Academic Press.

    Original languageEnglish (US)
    Pages (from-to)74-77
    Number of pages4
    JournalJournal of Surgical Research
    Volume89
    Issue number1
    DOIs
    StatePublished - Mar 2000

    Fingerprint

    Keloid
    Interleukin-6
    Fibroblasts
    Interferons
    Serum
    Cytokines
    Interstitial Nephritis
    Pulmonary Fibrosis
    Ribonucleases
    Autoimmune Diseases
    Intercellular Signaling Peptides and Proteins
    Collagen
    Genes

    Keywords

    • Fibroblasts
    • Interferon γ
    • Interleukin-6
    • Keloids

    ASJC Scopus subject areas

    • Surgery

    Cite this

    Elevated interleukin-6 expression in keloid fibroblasts. / Xue, Hui; McCauley, Robert L.; Zhang, Wenru.

    In: Journal of Surgical Research, Vol. 89, No. 1, 03.2000, p. 74-77.

    Research output: Contribution to journalArticle

    Xue, Hui ; McCauley, Robert L. ; Zhang, Wenru. / Elevated interleukin-6 expression in keloid fibroblasts. In: Journal of Surgical Research. 2000 ; Vol. 89, No. 1. pp. 74-77.
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