Elevated testosterone levels during rat pregnancy cause hypersensitivity to angiotensin II and attenuation of endothelium-dependent vasodilation in uterine arteries

Vijayakumar Chinnathambi, Chellakkan S. Blesson, Kathleen Vincent, George Saade, Gary Hankins, Chandra Yallampalli, Kunju Sathishkumar

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Elevated testosterone levels increase maternal blood pressure and decrease uterine blood flow in pregnancy, resulting in abnormal perinatal outcomes. We tested whether elevated testosterone alters uterine artery adaptations during pregnancy, and whether these alterations depend on endothelium-derived factors such as nitric oxide, endothelium-derived hyperpolarizing factor, and prostacyclin, or endothelium-independent mechanisms such as angiotensin II (Ang-II). Pregnant Sprague-Dawley rats were injected with vehicle (n=20) or testosterone propionate (0.5 mg/kg per day from gestation day 15 to 19; n=20). Plasma testosterone levels increased 2-fold in testosterone-injected rats compared with controls. Elevated testosterone significantly decreased placental and pup weights compared with controls. In endothelium-intact uterine arteries, contractile responses to thromboxane, phenylephrine, and Ang-II were greater in testosterone-treated rats compared with controls. In endothelium-denuded arteries, contractile responses to Ang-II (pD2=9.1±0.04 versus 8.7±0.04 in controls; P<0.05), but not thromboxane and phenylephrine, were greater in testosterone-treated rats. Ang-II type 1b receptor expression was increased, whereas Ang-II type 2 receptor was decreased in testosterone-exposed arteries. In endothelium-denuded arteries, relaxations to sodium nitroprusside were unaffected. Endothelium-dependent relaxation to acetylcholine was significantly lower in arteries from testosterone-treated dams (Emax=51.80±6.9% versus 91.98±1.4% in controls; P<0.05). The assessment of endothelial factors showed that nitric oxide-, endothelium-derived hyperpolarizing factor-, and prostacyclin-mediated relaxations were blunted in testosterone-treated dams. Endothelial nitric oxide synthase, small conductance calcium-activated potassium channel-3, and prostacyclin receptor expressions were significantly decreased in arteries from testosterone-treated dams. Hypoxia-inducible factor-1α, Ankrd37, and Egln were significantly increased in testosterone-exposed placentas. These results suggest that elevated maternal testosterone impairs uterine vascular function, which may lead to an increased vascular resistance and a decrease in uterine blood flow.

Original languageEnglish (US)
Pages (from-to)405-414
Number of pages10
JournalHypertension
Volume64
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Uterine Artery
Vasodilation
Angiotensin II
Endothelium
Testosterone
Hypersensitivity
Pregnancy
Arteries
Thromboxanes
Phenylephrine
Epoprostenol
Small-Conductance Calcium-Activated Potassium Channels
Nitric Oxide
Epoprostenol Receptors
Mothers
Angiotensin Type 2 Receptor
Testosterone Propionate
Hypoxia-Inducible Factor 1
Angiotensin Type 1 Receptor
Nitric Oxide Synthase Type III

Keywords

  • endothelium-dependent hyperpolarization factor
  • fetal development
  • nitric oxide
  • prostacyclin
  • uterine artery
  • vasoconstriction
  • vasodilation

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Elevated testosterone levels during rat pregnancy cause hypersensitivity to angiotensin II and attenuation of endothelium-dependent vasodilation in uterine arteries. / Chinnathambi, Vijayakumar; Blesson, Chellakkan S.; Vincent, Kathleen; Saade, George; Hankins, Gary; Yallampalli, Chandra; Sathishkumar, Kunju.

In: Hypertension, Vol. 64, No. 2, 2014, p. 405-414.

Research output: Contribution to journalArticle

Chinnathambi, Vijayakumar ; Blesson, Chellakkan S. ; Vincent, Kathleen ; Saade, George ; Hankins, Gary ; Yallampalli, Chandra ; Sathishkumar, Kunju. / Elevated testosterone levels during rat pregnancy cause hypersensitivity to angiotensin II and attenuation of endothelium-dependent vasodilation in uterine arteries. In: Hypertension. 2014 ; Vol. 64, No. 2. pp. 405-414.
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