Abstract
The inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin-1-beta (IL-1β), have been associated with accelerated metabolism and protein turnover following exogenous administration in normal humans. We hypothesized that these inflammatory cytokines might contribute to the weight-losing process in patients with chronic obstructive pulmonary disease (COPD). COPD patients were identified prospectively as 'weight losers' (WL; n = 10) if they reported > 5% weight loss during the preceding year or as 'weight stable' (W5; n = 10) if their body weight fluctuated ≤ 5%. Age- matched healthy volunteers were selected as the control group (C; n = 13). Monocytes were isolated from a peripheral blood sample, cultured, and exposed to lipopolysaccharide (LPS). The concentration of TNF-α and IL-1β in the monocyte supernatant was measured using a four layer enhanced ELISA. No significant difference in LPS-stimulated IL-1β production was found in the three study populations. However, LPS-stimulated TNF-α production (mean [range] ng/ml) by monocytes was significantly higher in the WL COPD patients (20.2 [6.3 to 44.8]), compared with WS patients (6.9 [1.5 to 16.6]), and C subjects (5.7 [0 to 61.8]). This difference was not maintained at 6 mo follow-up in the absence of ongoing weight loss. Definition of a causal relationship between TNF-α production and weight loss will require further understanding of the relationship between energy metabolism and TNF-α production in these patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 633-637 |
| Number of pages | 5 |
| Journal | American Journal of Respiratory and Critical Care Medicine |
| Volume | 153 |
| Issue number | 2 |
| State | Published - 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Elevated TNF-α production by peripheral blood monocytes of weight-losing COPD patients. / De Godoy, Irma; Donahoe, Michael; Calhoun, William; Mancino, Juliet; Rogers, Robert M.
In: American Journal of Respiratory and Critical Care Medicine, Vol. 153, No. 2, 1996, p. 633-637.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Elevated TNF-α production by peripheral blood monocytes of weight-losing COPD patients
AU - De Godoy, Irma
AU - Donahoe, Michael
AU - Calhoun, William
AU - Mancino, Juliet
AU - Rogers, Robert M.
PY - 1996
Y1 - 1996
N2 - The inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin-1-beta (IL-1β), have been associated with accelerated metabolism and protein turnover following exogenous administration in normal humans. We hypothesized that these inflammatory cytokines might contribute to the weight-losing process in patients with chronic obstructive pulmonary disease (COPD). COPD patients were identified prospectively as 'weight losers' (WL; n = 10) if they reported > 5% weight loss during the preceding year or as 'weight stable' (W5; n = 10) if their body weight fluctuated ≤ 5%. Age- matched healthy volunteers were selected as the control group (C; n = 13). Monocytes were isolated from a peripheral blood sample, cultured, and exposed to lipopolysaccharide (LPS). The concentration of TNF-α and IL-1β in the monocyte supernatant was measured using a four layer enhanced ELISA. No significant difference in LPS-stimulated IL-1β production was found in the three study populations. However, LPS-stimulated TNF-α production (mean [range] ng/ml) by monocytes was significantly higher in the WL COPD patients (20.2 [6.3 to 44.8]), compared with WS patients (6.9 [1.5 to 16.6]), and C subjects (5.7 [0 to 61.8]). This difference was not maintained at 6 mo follow-up in the absence of ongoing weight loss. Definition of a causal relationship between TNF-α production and weight loss will require further understanding of the relationship between energy metabolism and TNF-α production in these patients.
AB - The inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin-1-beta (IL-1β), have been associated with accelerated metabolism and protein turnover following exogenous administration in normal humans. We hypothesized that these inflammatory cytokines might contribute to the weight-losing process in patients with chronic obstructive pulmonary disease (COPD). COPD patients were identified prospectively as 'weight losers' (WL; n = 10) if they reported > 5% weight loss during the preceding year or as 'weight stable' (W5; n = 10) if their body weight fluctuated ≤ 5%. Age- matched healthy volunteers were selected as the control group (C; n = 13). Monocytes were isolated from a peripheral blood sample, cultured, and exposed to lipopolysaccharide (LPS). The concentration of TNF-α and IL-1β in the monocyte supernatant was measured using a four layer enhanced ELISA. No significant difference in LPS-stimulated IL-1β production was found in the three study populations. However, LPS-stimulated TNF-α production (mean [range] ng/ml) by monocytes was significantly higher in the WL COPD patients (20.2 [6.3 to 44.8]), compared with WS patients (6.9 [1.5 to 16.6]), and C subjects (5.7 [0 to 61.8]). This difference was not maintained at 6 mo follow-up in the absence of ongoing weight loss. Definition of a causal relationship between TNF-α production and weight loss will require further understanding of the relationship between energy metabolism and TNF-α production in these patients.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029670951&partnerID=8YFLogxK
M3 - Article
C2 - 8564110
AN - SCOPUS:0029670951
VL - 153
SP - 633
EP - 637
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
SN - 1073-449X
IS - 2
ER -