ELISA and neutralization methods to measure anti-west Nile virus antibody responses

Brian M. Friedrich, David W C Beasley

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Measurements of humoral immune responses to West Nile virus (WNV) infection in mouse or other animal models are valuable components of basic laboratory investigations to assess immunogenicity of candidate vaccines or to evaluate seroconversion following challenge with WNV. Here, we outline the steps for screening or titrating of total antibodies by indirect enzyme linked immunosorbent assay (ELISA) as well as assessment of neutralizing antibody titers by immunofocus detection.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages129-141
Number of pages13
Volume1435
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1435
ISSN (Print)10643745

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Keywords

  • Antibody titration
  • ELISA
  • FRNT
  • Humoral immunity
  • Immunofocus
  • Neutralization
  • West Nile virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Friedrich, B. M., & Beasley, D. W. C. (2016). ELISA and neutralization methods to measure anti-west Nile virus antibody responses. In Methods in Molecular Biology (Vol. 1435, pp. 129-141). (Methods in Molecular Biology; Vol. 1435). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3670-0_11