ELISA and neutralization methods to measure anti-west Nile virus antibody responses

Brian M. Friedrich, David Beasley

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Measurements of humoral immune responses to West Nile virus (WNV) infection in mouse or other animal models are valuable components of basic laboratory investigations to assess immunogenicity of candidate vaccines or to evaluate seroconversion following challenge with WNV. Here, we outline the steps for screening or titrating of total antibodies by indirect enzyme linked immunosorbent assay (ELISA) as well as assessment of neutralizing antibody titers by immunofocus detection.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages129-141
Number of pages13
Volume1435
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1435
ISSN (Print)10643745

Fingerprint

West Nile virus
Antibody Formation
Enzyme-Linked Immunosorbent Assay
Virus Diseases
Humoral Immunity
Neutralizing Antibodies
Animal Models
Antibodies
Vaccine Immunogenicity
Seroconversion

Keywords

  • Antibody titration
  • ELISA
  • FRNT
  • Humoral immunity
  • Immunofocus
  • Neutralization
  • West Nile virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Friedrich, B. M., & Beasley, D. (2016). ELISA and neutralization methods to measure anti-west Nile virus antibody responses. In Methods in Molecular Biology (Vol. 1435, pp. 129-141). (Methods in Molecular Biology; Vol. 1435). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3670-0_11

ELISA and neutralization methods to measure anti-west Nile virus antibody responses. / Friedrich, Brian M.; Beasley, David.

Methods in Molecular Biology. Vol. 1435 Humana Press Inc., 2016. p. 129-141 (Methods in Molecular Biology; Vol. 1435).

Research output: Chapter in Book/Report/Conference proceedingChapter

Friedrich, BM & Beasley, D 2016, ELISA and neutralization methods to measure anti-west Nile virus antibody responses. in Methods in Molecular Biology. vol. 1435, Methods in Molecular Biology, vol. 1435, Humana Press Inc., pp. 129-141. https://doi.org/10.1007/978-1-4939-3670-0_11
Friedrich BM, Beasley D. ELISA and neutralization methods to measure anti-west Nile virus antibody responses. In Methods in Molecular Biology. Vol. 1435. Humana Press Inc. 2016. p. 129-141. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3670-0_11
Friedrich, Brian M. ; Beasley, David. / ELISA and neutralization methods to measure anti-west Nile virus antibody responses. Methods in Molecular Biology. Vol. 1435 Humana Press Inc., 2016. pp. 129-141 (Methods in Molecular Biology).
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