ELISA methods for the detection of ebolavirus infection

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Ebola viruses are high-priority pathogens first discovered in rural Africa associated with sporadic outbreaks of severe hemorrhagic disease in humans and nonhuman primates. Little is known about the disease ecology or the prevalence of past exposure of human populations to any of the five species of the genus Ebolavirus. The use of immunologic means of detection for either virus antigens or the host’s immune response to antigen associated with prior infections offers a powerful approach at understanding the epidemiology and epizootiology of these agents. Here we describe methods for preparing antigen detection sandwich enzyme-linked immunosorbent assays (ELISAs) as well as IgG and IgM ELISAs for the detection of ebolavirus antigens or antibodies in biological samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages363-372
Number of pages10
Volume1628
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1628
ISSN (Print)1064-3745

Fingerprint

Ebolavirus
Enzyme-Linked Immunosorbent Assay
Antigens
Infection
Histocompatibility Antigens Class II
Ecology
Primates
Disease Outbreaks
Immunoglobulin M
Epidemiology
Immunoglobulin G
Viruses
Antibodies
Population

Keywords

  • Detection
  • Diagnostics
  • Ebola
  • ELISA
  • Filovirus
  • Surveillance

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Cross, R., & Ksiazek, T. (2017). ELISA methods for the detection of ebolavirus infection. In Methods in Molecular Biology (Vol. 1628, pp. 363-372). (Methods in Molecular Biology; Vol. 1628). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7116-9_29

ELISA methods for the detection of ebolavirus infection. / Cross, Robert; Ksiazek, Thomas.

Methods in Molecular Biology. Vol. 1628 Humana Press Inc., 2017. p. 363-372 (Methods in Molecular Biology; Vol. 1628).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cross, R & Ksiazek, T 2017, ELISA methods for the detection of ebolavirus infection. in Methods in Molecular Biology. vol. 1628, Methods in Molecular Biology, vol. 1628, Humana Press Inc., pp. 363-372. https://doi.org/10.1007/978-1-4939-7116-9_29
Cross R, Ksiazek T. ELISA methods for the detection of ebolavirus infection. In Methods in Molecular Biology. Vol. 1628. Humana Press Inc. 2017. p. 363-372. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7116-9_29
Cross, Robert ; Ksiazek, Thomas. / ELISA methods for the detection of ebolavirus infection. Methods in Molecular Biology. Vol. 1628 Humana Press Inc., 2017. pp. 363-372 (Methods in Molecular Biology).
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