Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approaches

Cynthia S. Goldsmith, Toni Whistler, Pierre E. Rollin, Thomas Ksiazek, Paul A. Rota, William J. Bellini, Peter Daszak, K. T. Wong, Wun Ju Shieh, Sherif R. Zaki

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Nipah virus, which was first recognized during an outbreak of encephalitis with high mortality in Peninsular Malaysia during 1998-1999, is most closely related to Hendra virus, another emergent paramyxovirus first recognized in Australia in 1994. We have studied the morphologic features of Nipah virus in infected Vero E6 cells and human brain by using standard and immunogold electron microscopy and ultrastructural in situ hybridization. Nipah virions are enveloped particles composed of a tangle of filamentous nucleocapsids and measured as large as 1900 nm in diameter. The nucleocapsids measured up to 1.67 μm in length and had the herringbone structure characteristic for paramyxoviruses. Cellular infection was associated with multinucleation, intracytoplasmic nucleocapsid inclusions (NCIs), and long cytoplasmic tubules. Previously undescribed for other members of the family Paramyxoviridae, infected cells also contained an inclusion formed of reticular structures. Ultrastructural ISH studies suggest these inclusions play an important role in the transcription process.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalVirus Research
Volume92
Issue number1
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

Fingerprint

Nipah Virus
Nucleocapsid
Virus Replication
Morphogenesis
Hendra Virus
Paramyxoviridae
Vero Cells
Malaysia
Inclusion Bodies
Encephalitis
Virion
In Situ Hybridization
Disease Outbreaks
Electron Microscopy
Mortality
Brain
Infection

Keywords

  • Electron microscopy
  • Encephalitis
  • Henipavirus
  • Immunogold labeling
  • In situ hybridization
  • Nipah virus
  • Paramyxoviridae
  • Replication complex
  • Ultrastructure
  • Zoonotic

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Goldsmith, C. S., Whistler, T., Rollin, P. E., Ksiazek, T., Rota, P. A., Bellini, W. J., ... Zaki, S. R. (2003). Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approaches. Virus Research, 92(1), 89-98. https://doi.org/10.1016/S0168-1702(02)00323-4

Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approaches. / Goldsmith, Cynthia S.; Whistler, Toni; Rollin, Pierre E.; Ksiazek, Thomas; Rota, Paul A.; Bellini, William J.; Daszak, Peter; Wong, K. T.; Shieh, Wun Ju; Zaki, Sherif R.

In: Virus Research, Vol. 92, No. 1, 01.03.2003, p. 89-98.

Research output: Contribution to journalArticle

Goldsmith, CS, Whistler, T, Rollin, PE, Ksiazek, T, Rota, PA, Bellini, WJ, Daszak, P, Wong, KT, Shieh, WJ & Zaki, SR 2003, 'Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approaches', Virus Research, vol. 92, no. 1, pp. 89-98. https://doi.org/10.1016/S0168-1702(02)00323-4
Goldsmith, Cynthia S. ; Whistler, Toni ; Rollin, Pierre E. ; Ksiazek, Thomas ; Rota, Paul A. ; Bellini, William J. ; Daszak, Peter ; Wong, K. T. ; Shieh, Wun Ju ; Zaki, Sherif R. / Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approaches. In: Virus Research. 2003 ; Vol. 92, No. 1. pp. 89-98.
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