Elucidation of the effects of lipoperoxidation on the mitochondrial electron transport chain using yeast mitochondria with manipulated fatty acid content

Christian Cortés-Rojo, Elizabeth Calderón-Cortés, Mónica Clemente-Guerrero, Mirella Estrada-Villagómez, Salvador Manzo-Avalos, Ricardo Mejía-Zepeda, Istvan Boldogh, Alfredo Saavedra-Molina

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Lipoperoxidative damage to the respiratory chain proteins may account for disruption in mitochondrial electron transport chain (ETC) function and could lead to an augment in the production of reactive oxygen species (ROS). To test this hypothesis, we investigated the effects of lipoperoxidation on ETC function and cytochromes spectra of Saccharomyces cerevisiae mitochondria. We compared the effects of Fe2+ treatment on mitochondria isolated from yeast with native (lipoperoxidation-resistant) and modified (lipoperoxidation- sensitive) fatty acid composition. Augmented sensitivity to oxidative stress was observed in the complex III-complex IV segment of the ETC. Lipoperoxidation did not alter the cytochromes content. Under lipoperoxidative conditions, cytochrome c reduction by succinate was almost totally eliminated by superoxide dismutase and stigmatellin. Our results suggest that lipoperoxidation impairs electron transfer mainly at cytochrome b in complex III, which leads to increased resistance to antimycin A and ROS generation due to an electron leak at the level of the QO site of complex III.

Original languageEnglish (US)
Pages (from-to)15-28
Number of pages14
JournalJournal of Bioenergetics and Biomembranes
Volume41
Issue number1
DOIs
StatePublished - Feb 2009

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Electron Transport Complex III
Electron Transport
Mitochondria
Fatty Acids
Yeasts
Cytochromes
Reactive Oxygen Species
Electrons
Antimycin A
Cytochromes b
Succinic Acid
Electron Transport Complex IV
Cytochromes c
Superoxide Dismutase
Saccharomyces cerevisiae
Oxidative Stress
Proteins

Keywords

  • Cytochromes
  • Electron transport chain
  • Iron
  • Lipoperoxidation
  • Yeast mitochondria

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Cortés-Rojo, C., Calderón-Cortés, E., Clemente-Guerrero, M., Estrada-Villagómez, M., Manzo-Avalos, S., Mejía-Zepeda, R., ... Saavedra-Molina, A. (2009). Elucidation of the effects of lipoperoxidation on the mitochondrial electron transport chain using yeast mitochondria with manipulated fatty acid content. Journal of Bioenergetics and Biomembranes, 41(1), 15-28. https://doi.org/10.1007/s10863-009-9200-3

Elucidation of the effects of lipoperoxidation on the mitochondrial electron transport chain using yeast mitochondria with manipulated fatty acid content. / Cortés-Rojo, Christian; Calderón-Cortés, Elizabeth; Clemente-Guerrero, Mónica; Estrada-Villagómez, Mirella; Manzo-Avalos, Salvador; Mejía-Zepeda, Ricardo; Boldogh, Istvan; Saavedra-Molina, Alfredo.

In: Journal of Bioenergetics and Biomembranes, Vol. 41, No. 1, 02.2009, p. 15-28.

Research output: Contribution to journalArticle

Cortés-Rojo, C, Calderón-Cortés, E, Clemente-Guerrero, M, Estrada-Villagómez, M, Manzo-Avalos, S, Mejía-Zepeda, R, Boldogh, I & Saavedra-Molina, A 2009, 'Elucidation of the effects of lipoperoxidation on the mitochondrial electron transport chain using yeast mitochondria with manipulated fatty acid content', Journal of Bioenergetics and Biomembranes, vol. 41, no. 1, pp. 15-28. https://doi.org/10.1007/s10863-009-9200-3
Cortés-Rojo, Christian ; Calderón-Cortés, Elizabeth ; Clemente-Guerrero, Mónica ; Estrada-Villagómez, Mirella ; Manzo-Avalos, Salvador ; Mejía-Zepeda, Ricardo ; Boldogh, Istvan ; Saavedra-Molina, Alfredo. / Elucidation of the effects of lipoperoxidation on the mitochondrial electron transport chain using yeast mitochondria with manipulated fatty acid content. In: Journal of Bioenergetics and Biomembranes. 2009 ; Vol. 41, No. 1. pp. 15-28.
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