Embryonal mitochondrial DNA: relationship to embryo quality and transfer outcomes

Amber M. Klimczak, Luis Pacheco, Kelsey Lewis, Niloofar Massahi, Jon P. Richards, William G. Kearns, Antonio Saad, John R. Crochet

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: The aim was to study the association between embryonal mitochondrial DNA (mtDNA) content and embryo quality and implantation outcomes. Methods: A retrospective chart review was performed with data collected from a private IVF center database. The study population included female infertility patients with ages ranging from 31 to 38 years old, and the main outcome measures were embryo quality and transfer outcomes. Results: From a total of 1510 blastocyst biopsies, the majority of embryos consisted of grade 1 (High), followed by grade 2 (mid), and grade 3 (poor). Embryos with higher mtDNA content were found to be of poorer quality (grade 3) relative to grades 1 and 2 (P = 0.003). Using a logistic model, mtDNA best predicted lowest and highest grades, but not mid-grade embryos. There was no correlation between mtDNA content and the subjects’ age (R2 = 0.0018). In an analysis of only euploid embryos (N = 717), there was no longer an association between mtDNA content and embryo quality (P = 0.834). There was no difference in mtDNA content between groups of embryos that did and did not implant (P = 0.53). There was also no association noted between mtDNA content and ongoing pregnancy. Compared to day 6, day 5 blastocysts contain significantly higher amounts of mtDNA (P = 0.0005), lower rates of aneuploidy (P < 0.001), and were more likely to be high-quality blastocysts (grade 1) (P < 0.001). Conclusion: Although the mtDNA content shows some association to the morphologic grade of an embryo, this association does not persist in an analysis of only euploid embryos. Mitochondrial DNA content also does not appear to be associated with implantation or ongoing pregnancy. Day 5 blastocysts have significantly higher mtDNA content compared to day 6 blastocysts.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalJournal of Assisted Reproduction and Genetics
DOIs
StateAccepted/In press - Mar 5 2018

Fingerprint

Embryo Transfer
Mitochondrial DNA
Embryonic Structures
Blastocyst
Female Infertility
Pregnancy
Aneuploidy
Logistic Models
Outcome Assessment (Health Care)
Databases
Biopsy

Keywords

  • Embryo grade
  • Embryo quality
  • IVF
  • Mitochondrial DNA
  • PGS

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

Embryonal mitochondrial DNA : relationship to embryo quality and transfer outcomes. / Klimczak, Amber M.; Pacheco, Luis; Lewis, Kelsey; Massahi, Niloofar; Richards, Jon P.; Kearns, William G.; Saad, Antonio; Crochet, John R.

In: Journal of Assisted Reproduction and Genetics, 05.03.2018, p. 1-7.

Research output: Contribution to journalArticle

Klimczak, Amber M. ; Pacheco, Luis ; Lewis, Kelsey ; Massahi, Niloofar ; Richards, Jon P. ; Kearns, William G. ; Saad, Antonio ; Crochet, John R. / Embryonal mitochondrial DNA : relationship to embryo quality and transfer outcomes. In: Journal of Assisted Reproduction and Genetics. 2018 ; pp. 1-7.
@article{6664f9f8bda14cf99149ca907c0313a7,
title = "Embryonal mitochondrial DNA: relationship to embryo quality and transfer outcomes",
abstract = "Purpose: The aim was to study the association between embryonal mitochondrial DNA (mtDNA) content and embryo quality and implantation outcomes. Methods: A retrospective chart review was performed with data collected from a private IVF center database. The study population included female infertility patients with ages ranging from 31 to 38 years old, and the main outcome measures were embryo quality and transfer outcomes. Results: From a total of 1510 blastocyst biopsies, the majority of embryos consisted of grade 1 (High), followed by grade 2 (mid), and grade 3 (poor). Embryos with higher mtDNA content were found to be of poorer quality (grade 3) relative to grades 1 and 2 (P = 0.003). Using a logistic model, mtDNA best predicted lowest and highest grades, but not mid-grade embryos. There was no correlation between mtDNA content and the subjects’ age (R2 = 0.0018). In an analysis of only euploid embryos (N = 717), there was no longer an association between mtDNA content and embryo quality (P = 0.834). There was no difference in mtDNA content between groups of embryos that did and did not implant (P = 0.53). There was also no association noted between mtDNA content and ongoing pregnancy. Compared to day 6, day 5 blastocysts contain significantly higher amounts of mtDNA (P = 0.0005), lower rates of aneuploidy (P < 0.001), and were more likely to be high-quality blastocysts (grade 1) (P < 0.001). Conclusion: Although the mtDNA content shows some association to the morphologic grade of an embryo, this association does not persist in an analysis of only euploid embryos. Mitochondrial DNA content also does not appear to be associated with implantation or ongoing pregnancy. Day 5 blastocysts have significantly higher mtDNA content compared to day 6 blastocysts.",
keywords = "Embryo grade, Embryo quality, IVF, Mitochondrial DNA, PGS",
author = "Klimczak, {Amber M.} and Luis Pacheco and Kelsey Lewis and Niloofar Massahi and Richards, {Jon P.} and Kearns, {William G.} and Antonio Saad and Crochet, {John R.}",
year = "2018",
month = "3",
day = "5",
doi = "10.1007/s10815-018-1147-z",
language = "English (US)",
pages = "1--7",
journal = "Journal of in Vitro Fertilization and Embryo Transfer",
issn = "0740-7769",
publisher = "Springer New York",

}

TY - JOUR

T1 - Embryonal mitochondrial DNA

T2 - relationship to embryo quality and transfer outcomes

AU - Klimczak, Amber M.

AU - Pacheco, Luis

AU - Lewis, Kelsey

AU - Massahi, Niloofar

AU - Richards, Jon P.

AU - Kearns, William G.

AU - Saad, Antonio

AU - Crochet, John R.

PY - 2018/3/5

Y1 - 2018/3/5

N2 - Purpose: The aim was to study the association between embryonal mitochondrial DNA (mtDNA) content and embryo quality and implantation outcomes. Methods: A retrospective chart review was performed with data collected from a private IVF center database. The study population included female infertility patients with ages ranging from 31 to 38 years old, and the main outcome measures were embryo quality and transfer outcomes. Results: From a total of 1510 blastocyst biopsies, the majority of embryos consisted of grade 1 (High), followed by grade 2 (mid), and grade 3 (poor). Embryos with higher mtDNA content were found to be of poorer quality (grade 3) relative to grades 1 and 2 (P = 0.003). Using a logistic model, mtDNA best predicted lowest and highest grades, but not mid-grade embryos. There was no correlation between mtDNA content and the subjects’ age (R2 = 0.0018). In an analysis of only euploid embryos (N = 717), there was no longer an association between mtDNA content and embryo quality (P = 0.834). There was no difference in mtDNA content between groups of embryos that did and did not implant (P = 0.53). There was also no association noted between mtDNA content and ongoing pregnancy. Compared to day 6, day 5 blastocysts contain significantly higher amounts of mtDNA (P = 0.0005), lower rates of aneuploidy (P < 0.001), and were more likely to be high-quality blastocysts (grade 1) (P < 0.001). Conclusion: Although the mtDNA content shows some association to the morphologic grade of an embryo, this association does not persist in an analysis of only euploid embryos. Mitochondrial DNA content also does not appear to be associated with implantation or ongoing pregnancy. Day 5 blastocysts have significantly higher mtDNA content compared to day 6 blastocysts.

AB - Purpose: The aim was to study the association between embryonal mitochondrial DNA (mtDNA) content and embryo quality and implantation outcomes. Methods: A retrospective chart review was performed with data collected from a private IVF center database. The study population included female infertility patients with ages ranging from 31 to 38 years old, and the main outcome measures were embryo quality and transfer outcomes. Results: From a total of 1510 blastocyst biopsies, the majority of embryos consisted of grade 1 (High), followed by grade 2 (mid), and grade 3 (poor). Embryos with higher mtDNA content were found to be of poorer quality (grade 3) relative to grades 1 and 2 (P = 0.003). Using a logistic model, mtDNA best predicted lowest and highest grades, but not mid-grade embryos. There was no correlation between mtDNA content and the subjects’ age (R2 = 0.0018). In an analysis of only euploid embryos (N = 717), there was no longer an association between mtDNA content and embryo quality (P = 0.834). There was no difference in mtDNA content between groups of embryos that did and did not implant (P = 0.53). There was also no association noted between mtDNA content and ongoing pregnancy. Compared to day 6, day 5 blastocysts contain significantly higher amounts of mtDNA (P = 0.0005), lower rates of aneuploidy (P < 0.001), and were more likely to be high-quality blastocysts (grade 1) (P < 0.001). Conclusion: Although the mtDNA content shows some association to the morphologic grade of an embryo, this association does not persist in an analysis of only euploid embryos. Mitochondrial DNA content also does not appear to be associated with implantation or ongoing pregnancy. Day 5 blastocysts have significantly higher mtDNA content compared to day 6 blastocysts.

KW - Embryo grade

KW - Embryo quality

KW - IVF

KW - Mitochondrial DNA

KW - PGS

UR - http://www.scopus.com/inward/record.url?scp=85045026628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045026628&partnerID=8YFLogxK

U2 - 10.1007/s10815-018-1147-z

DO - 10.1007/s10815-018-1147-z

M3 - Article

C2 - 29508122

AN - SCOPUS:85045026628

SP - 1

EP - 7

JO - Journal of in Vitro Fertilization and Embryo Transfer

JF - Journal of in Vitro Fertilization and Embryo Transfer

SN - 0740-7769

ER -