Embryonic hemoglobins are expressed in definitive cells

H. Y. Luo, X. L. Liang, C. Frye, M. Wonio, Gary Hankins, D. H K Chui, B. P. Alter

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Human embryonic ζ and ε globin chains are synthesized in yolk sac- derived primitive erythroid cells, and decrease rapidly during definitive erythropoiesis. Examination of and ε globin expression at the cellular level using dual-color immunofluorescence staining with specific monoclonal antibodies showed that embryonic globin proteins are present in definitive erythroid cells. More than half of fetal erythrocytes were positive for ζ and ~5% for ε globin. Approximately one third of newborn red blood cells were ζ-positive and less than 1% ε-positive. Adult erythrocytes did not have embryonic globins. Erythroblasts that developed in liquid cultures also contained embryonic globin in amounts which declined with ontogenic age, and the proportion of positive cells in vitro was less than in the comparable erythrocytes that developed in vivo. Thus, embryonic globin chains are synthesized in definitive erythroid cells and decrease with ontogeny. Modulation of embryonic globin gene expression is not solely due to a switch from primitive to definitive erythropoiesis.

Original languageEnglish (US)
Pages (from-to)359-361
Number of pages3
JournalBlood
Volume94
Issue number1
StatePublished - Jul 1 1999
Externally publishedYes

Fingerprint

Globins
Hemoglobins
Erythroid Cells
Erythrocytes
Erythropoiesis
Cells
Erythroblasts
Yolk Sac
Gene expression
Fluorescent Antibody Technique
Blood
Color
Monoclonal Antibodies
Switches
Modulation
Staining and Labeling
Gene Expression
Liquids

ASJC Scopus subject areas

  • Hematology

Cite this

Luo, H. Y., Liang, X. L., Frye, C., Wonio, M., Hankins, G., Chui, D. H. K., & Alter, B. P. (1999). Embryonic hemoglobins are expressed in definitive cells. Blood, 94(1), 359-361.

Embryonic hemoglobins are expressed in definitive cells. / Luo, H. Y.; Liang, X. L.; Frye, C.; Wonio, M.; Hankins, Gary; Chui, D. H K; Alter, B. P.

In: Blood, Vol. 94, No. 1, 01.07.1999, p. 359-361.

Research output: Contribution to journalArticle

Luo, HY, Liang, XL, Frye, C, Wonio, M, Hankins, G, Chui, DHK & Alter, BP 1999, 'Embryonic hemoglobins are expressed in definitive cells', Blood, vol. 94, no. 1, pp. 359-361.
Luo HY, Liang XL, Frye C, Wonio M, Hankins G, Chui DHK et al. Embryonic hemoglobins are expressed in definitive cells. Blood. 1999 Jul 1;94(1):359-361.
Luo, H. Y. ; Liang, X. L. ; Frye, C. ; Wonio, M. ; Hankins, Gary ; Chui, D. H K ; Alter, B. P. / Embryonic hemoglobins are expressed in definitive cells. In: Blood. 1999 ; Vol. 94, No. 1. pp. 359-361.
@article{3a9bf9ab656c4beca82a398f0eaaa81a,
title = "Embryonic hemoglobins are expressed in definitive cells",
abstract = "Human embryonic ζ and ε globin chains are synthesized in yolk sac- derived primitive erythroid cells, and decrease rapidly during definitive erythropoiesis. Examination of and ε globin expression at the cellular level using dual-color immunofluorescence staining with specific monoclonal antibodies showed that embryonic globin proteins are present in definitive erythroid cells. More than half of fetal erythrocytes were positive for ζ and ~5{\%} for ε globin. Approximately one third of newborn red blood cells were ζ-positive and less than 1{\%} ε-positive. Adult erythrocytes did not have embryonic globins. Erythroblasts that developed in liquid cultures also contained embryonic globin in amounts which declined with ontogenic age, and the proportion of positive cells in vitro was less than in the comparable erythrocytes that developed in vivo. Thus, embryonic globin chains are synthesized in definitive erythroid cells and decrease with ontogeny. Modulation of embryonic globin gene expression is not solely due to a switch from primitive to definitive erythropoiesis.",
author = "Luo, {H. Y.} and Liang, {X. L.} and C. Frye and M. Wonio and Gary Hankins and Chui, {D. H K} and Alter, {B. P.}",
year = "1999",
month = "7",
day = "1",
language = "English (US)",
volume = "94",
pages = "359--361",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

TY - JOUR

T1 - Embryonic hemoglobins are expressed in definitive cells

AU - Luo, H. Y.

AU - Liang, X. L.

AU - Frye, C.

AU - Wonio, M.

AU - Hankins, Gary

AU - Chui, D. H K

AU - Alter, B. P.

PY - 1999/7/1

Y1 - 1999/7/1

N2 - Human embryonic ζ and ε globin chains are synthesized in yolk sac- derived primitive erythroid cells, and decrease rapidly during definitive erythropoiesis. Examination of and ε globin expression at the cellular level using dual-color immunofluorescence staining with specific monoclonal antibodies showed that embryonic globin proteins are present in definitive erythroid cells. More than half of fetal erythrocytes were positive for ζ and ~5% for ε globin. Approximately one third of newborn red blood cells were ζ-positive and less than 1% ε-positive. Adult erythrocytes did not have embryonic globins. Erythroblasts that developed in liquid cultures also contained embryonic globin in amounts which declined with ontogenic age, and the proportion of positive cells in vitro was less than in the comparable erythrocytes that developed in vivo. Thus, embryonic globin chains are synthesized in definitive erythroid cells and decrease with ontogeny. Modulation of embryonic globin gene expression is not solely due to a switch from primitive to definitive erythropoiesis.

AB - Human embryonic ζ and ε globin chains are synthesized in yolk sac- derived primitive erythroid cells, and decrease rapidly during definitive erythropoiesis. Examination of and ε globin expression at the cellular level using dual-color immunofluorescence staining with specific monoclonal antibodies showed that embryonic globin proteins are present in definitive erythroid cells. More than half of fetal erythrocytes were positive for ζ and ~5% for ε globin. Approximately one third of newborn red blood cells were ζ-positive and less than 1% ε-positive. Adult erythrocytes did not have embryonic globins. Erythroblasts that developed in liquid cultures also contained embryonic globin in amounts which declined with ontogenic age, and the proportion of positive cells in vitro was less than in the comparable erythrocytes that developed in vivo. Thus, embryonic globin chains are synthesized in definitive erythroid cells and decrease with ontogeny. Modulation of embryonic globin gene expression is not solely due to a switch from primitive to definitive erythropoiesis.

UR - http://www.scopus.com/inward/record.url?scp=0033169050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033169050&partnerID=8YFLogxK

M3 - Article

VL - 94

SP - 359

EP - 361

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -