Enantioseparation and detection of (R)-2-hydroxyglutarate and (S)-2-hydroxyglutarate by chiral gas chromatography–triple quadrupole mass spectrometry

Shinji K. Strain; Morris D. Groves; Mark R. Emmett

doi:10.1007/978-1-0716-0864-7_8

Enantioseparation and detection of (R)-2-hydroxyglutarate and (S)-2-hydroxyglutarate by chiral gas chromatography–triple quadrupole mass spectrometry

Shinji K. Strain, Morris D. Groves, Mark R. Emmett

Biochemistry & Molecular Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

(R)-2-hydroxyglutarate (R-2-hg) has gained significant interest as a circulating biomarker for patients with cancers that harbor mutations in the isocitrate dehydrogenase (IDH) gene. R-2-hg detection is challenging as it must be separated from its enantiomer, (S)-2-hydroxyglutarate (S-2-hg). Here, we present a novel gas chromatography–tandem mass spectrometry method (GC-MS/MS) assay that can be used to separate and detect 2-hg enantiomers for clinical use.

Original language	English (US)
Title of host publication	Neuromethods
Publisher	Humana Press Inc.
Pages	89-100
Number of pages	12
DOIs	https://doi.org/10.1007/978-1-0716-0864-7_8
State	Published - 2021

Publication series

Name	Neuromethods
Volume	159
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

2-hydroxyglutarate enantiomer
Acute Myeloid Leukemia
Chiral Chromatography
Cholangiocarcinoma
Gas chromatography–tandem mass spectrometry
Glioma
Isocitrate dehydrogenase
Serum
Urine

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)
Psychiatry and Mental health

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10.1007/978-1-0716-0864-7_8

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title = "Enantioseparation and detection of (R)-2-hydroxyglutarate and (S)-2-hydroxyglutarate by chiral gas chromatography–triple quadrupole mass spectrometry",

abstract = "(R)-2-hydroxyglutarate (R-2-hg) has gained significant interest as a circulating biomarker for patients with cancers that harbor mutations in the isocitrate dehydrogenase (IDH) gene. R-2-hg detection is challenging as it must be separated from its enantiomer, (S)-2-hydroxyglutarate (S-2-hg). Here, we present a novel gas chromatography–tandem mass spectrometry method (GC-MS/MS) assay that can be used to separate and detect 2-hg enantiomers for clinical use.",

keywords = "2-hydroxyglutarate enantiomer, Acute Myeloid Leukemia, Chiral Chromatography, Cholangiocarcinoma, Gas chromatography–tandem mass spectrometry, Glioma, Isocitrate dehydrogenase, Serum, Urine",

author = "Strain, {Shinji K.} and Groves, {Morris D.} and Emmett, {Mark R.}",

note = "Funding Information: This work was supported by The Shimadzu Corporation and The Head for The Cure Foundation. SKS was supported by The Institute for Translational Sciences at The University of Texas Medical Branch, supported, in part, by a Clinical and Translational Science Award NRSA (TL1) Training Core (TL1TR001440) from the National Center for Advancing Translational Sciences, National Institutes of Health.",

year = "2021",

doi = "10.1007/978-1-0716-0864-7_8",

language = "English (US)",

series = "Neuromethods",

publisher = "Humana Press Inc.",

pages = "89--100",

booktitle = "Neuromethods",

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T1 - Enantioseparation and detection of (R)-2-hydroxyglutarate and (S)-2-hydroxyglutarate by chiral gas chromatography–triple quadrupole mass spectrometry

AU - Strain, Shinji K.

AU - Groves, Morris D.

AU - Emmett, Mark R.

N1 - Funding Information: This work was supported by The Shimadzu Corporation and The Head for The Cure Foundation. SKS was supported by The Institute for Translational Sciences at The University of Texas Medical Branch, supported, in part, by a Clinical and Translational Science Award NRSA (TL1) Training Core (TL1TR001440) from the National Center for Advancing Translational Sciences, National Institutes of Health.

PY - 2021

Y1 - 2021

N2 - (R)-2-hydroxyglutarate (R-2-hg) has gained significant interest as a circulating biomarker for patients with cancers that harbor mutations in the isocitrate dehydrogenase (IDH) gene. R-2-hg detection is challenging as it must be separated from its enantiomer, (S)-2-hydroxyglutarate (S-2-hg). Here, we present a novel gas chromatography–tandem mass spectrometry method (GC-MS/MS) assay that can be used to separate and detect 2-hg enantiomers for clinical use.

AB - (R)-2-hydroxyglutarate (R-2-hg) has gained significant interest as a circulating biomarker for patients with cancers that harbor mutations in the isocitrate dehydrogenase (IDH) gene. R-2-hg detection is challenging as it must be separated from its enantiomer, (S)-2-hydroxyglutarate (S-2-hg). Here, we present a novel gas chromatography–tandem mass spectrometry method (GC-MS/MS) assay that can be used to separate and detect 2-hg enantiomers for clinical use.

KW - 2-hydroxyglutarate enantiomer

KW - Acute Myeloid Leukemia

KW - Chiral Chromatography

KW - Cholangiocarcinoma

KW - Gas chromatography–tandem mass spectrometry

KW - Glioma

KW - Isocitrate dehydrogenase

KW - Serum

KW - Urine

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PB - Humana Press Inc.

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