Enantioseparation and detection of (R)-2-hydroxyglutarate and (S)-2-hydroxyglutarate by chiral gas chromatography–triple quadrupole mass spectrometry

Shinji K. Strain, Morris D. Groves, Mark R. Emmett

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

(R)-2-hydroxyglutarate (R-2-hg) has gained significant interest as a circulating biomarker for patients with cancers that harbor mutations in the isocitrate dehydrogenase (IDH) gene. R-2-hg detection is challenging as it must be separated from its enantiomer, (S)-2-hydroxyglutarate (S-2-hg). Here, we present a novel gas chromatography–tandem mass spectrometry method (GC-MS/MS) assay that can be used to separate and detect 2-hg enantiomers for clinical use.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages89-100
Number of pages12
DOIs
StatePublished - 2021
Externally publishedYes

Publication series

NameNeuromethods
Volume159
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • 2-hydroxyglutarate enantiomer
  • Acute Myeloid Leukemia
  • Chiral Chromatography
  • Cholangiocarcinoma
  • Gas chromatography–tandem mass spectrometry
  • Glioma
  • Isocitrate dehydrogenase
  • Serum
  • Urine

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience

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