Encapsulation of Adenovirus BMP2-Transduced Cells with PEGDA Hydrogels Allows Bone Formation in the Presence of Immune Response

Pedro Alvarez-Urena, Eleanor Davis, Corinne Sonnet, Gabrielle Henslee, Zbigniew Gugala, Edward V. Strecker, Laura J. Linscheid, Maude Cuchiara, Jennifer West, Alan Davis, Elizabeth Olmsted-Davis

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Gene therapy approaches have been difficult to implement due to pre-existing immunity against the virus used for delivery. To circumvent this problem, a cell-based approach was developed that avoided the use of free virus within the animal. However, even cells transduced in vitro with E1- to E3-deleted adenovirus encoding bone morphogenetic protein 2 (AdBMP2) resulted in the production of virus-neutralizing antibodies in mice. Furthermore, when mice received an intramuscular injection of nonencoding adenovirus (AdEmpty)-transduced cells, AdBMP2-transduced cells were unable to launch bone formation when an intramuscular injection of these BMP2-producing cells was delivered 1 week later. This phenomenon was not observed in NOD/SCID mice, and could be overcome in C57BL/6 mice by encapsulating the adenovirus-transduced cells in a nondegradable hydrogel poly(ethylene glycol) diacrylate (PEGDA). Data collectively suggest that PEGDA hydrogel encapsulation of AdBMP2-transduced cells prevents pre-existing immunity from suppressing BMP2-induced bone formation.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalTissue Engineering - Part A
Volume23
Issue number5-6
DOIs
StatePublished - Mar 2017

Keywords

  • bone formation
  • cell therapy
  • in vivo delivery system
  • priming

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

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    Alvarez-Urena, P., Davis, E., Sonnet, C., Henslee, G., Gugala, Z., Strecker, E. V., Linscheid, L. J., Cuchiara, M., West, J., Davis, A., & Olmsted-Davis, E. (2017). Encapsulation of Adenovirus BMP2-Transduced Cells with PEGDA Hydrogels Allows Bone Formation in the Presence of Immune Response. Tissue Engineering - Part A, 23(5-6), 177-184. https://doi.org/10.1089/ten.tea.2016.0277