Endogenous mast cell degranulation modulates cervical contractility in the guinea pig

Egle Bytautiene, Yuri P. Vedernikov, George Saade, Roberto Romero, Robert E. Garfield

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

OBJECTIVE: The purpose of this study was to investigate the effect of endogenous mast cell degranulation on the contractility of isolated cervical strips from nonpregnant and pregnant guinea pigs. STUDY DESIGN: Longitudinal cervical strips from nonpregnant and pregnant (mid and term) guinea pigs were used for isometric tension recording. Responses to the mast cell degranulating agent, compound 48/80, were compared in the absence or presence of different inhibitors and receptor antagonists. Concentration-response curves were obtained to histamine and 5-hydroxytryptamine in strips that were incubated with antagonists or solvent. RESULTS: Compound 48/80 and histamine significantly increased contractility of cervical strips in all 3 groups of animals. The inhibitor of mast cell degranulation significantly reduced responses to compound 48/80 and histamine-1 receptor antagonist reduced responses to histamine in all 3 groups. Histamine-1 receptor antagonist significantly inhibited responses to compound 48/80 in nonpregnant and mid pregnant guinea pigs. Histamine-2 receptor antagonist did not alter responses to compound 48/80 nor to histamine. The receptor antagonist 5-hydroxytryptamine-2 significantly inhibited cervical contractility that was induced by compound 48/80 in tissues from mid pregnant and term pregnant guinea pigs. Lipoxygenase inhibitor was effective in mid pregnant guinea pigs. Cyclooxygenase inhibitor, 5-hydroxytryptamine, and a combination of lipoxygenase and cyclooxygenase inhibitors had no effect on cervical contractility. CONCLUSION: The degranulation of mast cells releases histamine and other mediators that stimulate cervical contractility through histamine-1 receptors. Cervical infiltration and modulation of contractility by mast cells may play an important physiologic and/or pathologic role in the control of cervical function during pregnancy.

Original languageEnglish (US)
Pages (from-to)438-445
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume186
Issue number3
DOIs
StatePublished - 2002

Fingerprint

p-Methoxy-N-methylphenethylamine
Cell Degranulation
Mast Cells
Guinea Pigs
Histamine Receptors
Histamine
Lipoxygenase Inhibitors
Cyclooxygenase Inhibitors
Serotonin
Serotonin Antagonists
Histamine Release
Serotonin Receptors
Pregnancy

Keywords

  • Cervix
  • Contractility
  • Hypersensitivity
  • Mast cells
  • Pregnancy

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Endogenous mast cell degranulation modulates cervical contractility in the guinea pig. / Bytautiene, Egle; Vedernikov, Yuri P.; Saade, George; Romero, Roberto; Garfield, Robert E.

In: American Journal of Obstetrics and Gynecology, Vol. 186, No. 3, 2002, p. 438-445.

Research output: Contribution to journalArticle

Bytautiene, Egle ; Vedernikov, Yuri P. ; Saade, George ; Romero, Roberto ; Garfield, Robert E. / Endogenous mast cell degranulation modulates cervical contractility in the guinea pig. In: American Journal of Obstetrics and Gynecology. 2002 ; Vol. 186, No. 3. pp. 438-445.
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