Endogenously produced peroxynitrite induces the oxidation of mitochondrial and nuclear proteins in immunostimulated macrophages

Csaba Szabo, Michael O'Connor, Andrew L. Salzman

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Here we investigated the role of endogenous nitric oxide (NO) and peroxynitrite in the process of protein oxidation (as measured by the detection of 2,4-dinitrophenylhydrazine-reactive carbonyls) in immunostimulated macrophages. Immunostimulation of the macrophages by bacterial lipopolysaccharide and gamma-interferon (LPS/IFNγ) resulted in a marked increase in the oxidation of a large number of mitochondrial and nuclear proteins. The inhibitor of NO synthase, N(G)-methyl-L-arginine (3 mM), and the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis(4-benzoic acid)porphyrin (300 μM) both reduced the extent of protein oxidation in response to LPS/IFNγ. These results support the view that endogenously produced peroxynitrite induces protein oxidation in the mitochondria and nucleus of immunostimulated macrophages.

Original languageEnglish (US)
Pages (from-to)147-150
Number of pages4
JournalFEBS Letters
Volume409
Issue number2
DOIs
StatePublished - Jun 9 1997
Externally publishedYes

Fingerprint

Peroxynitrous Acid
Macrophages
Mitochondrial Proteins
Nuclear Proteins
Oxidation
Interferon-gamma
Lipopolysaccharides
Proteins
Nitric Oxide Synthase
Mitochondria
Superoxide Dismutase
Arginine
Immunization
Nitric Oxide

Keywords

  • Inflammation
  • Macrophage
  • Nitric oxide
  • Oxidation
  • Peroxynitrite
  • Protein
  • Shock
  • Superoxide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Endogenously produced peroxynitrite induces the oxidation of mitochondrial and nuclear proteins in immunostimulated macrophages. / Szabo, Csaba; O'Connor, Michael; Salzman, Andrew L.

In: FEBS Letters, Vol. 409, No. 2, 09.06.1997, p. 147-150.

Research output: Contribution to journalArticle

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