Endogenously produced peroxynitrite induces the oxidation of mitochondrial and nuclear proteins in immunostimulated macrophages

Csaba Szabó, Michael O'Connor, Andrew L. Salzman

    Research output: Contribution to journalArticle

    33 Scopus citations

    Abstract

    Here we investigated the role of endogenous nitric oxide (NO) and peroxynitrite in the process of protein oxidation (as measured by the detection of 2,4-dinitrophenylhydrazine-reactive carbonyls) in immunostimulated macrophages. Immunostimulation of the macrophages by bacterial lipopolysaccharide and gamma-interferon (LPS/IFNγ) resulted in a marked increase in the oxidation of a large number of mitochondrial and nuclear proteins. The inhibitor of NO synthase, N(G)-methyl-L-arginine (3 mM), and the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis(4-benzoic acid)porphyrin (300 μM) both reduced the extent of protein oxidation in response to LPS/IFNγ. These results support the view that endogenously produced peroxynitrite induces protein oxidation in the mitochondria and nucleus of immunostimulated macrophages.

    Original languageEnglish (US)
    Pages (from-to)147-150
    Number of pages4
    JournalFEBS Letters
    Volume409
    Issue number2
    DOIs
    StatePublished - Jun 9 1997

    Keywords

    • Inflammation
    • Macrophage
    • Nitric oxide
    • Oxidation
    • Peroxynitrite
    • Protein
    • Shock
    • Superoxide

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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