Endogenously produced peroxynitrite induces the oxidation of mitochondrial and nuclear proteins in immunostimulated macrophages

Csaba Szabó, Michael O'Connor, Andrew L. Salzman

    Research output: Contribution to journalArticlepeer-review

    33 Scopus citations

    Abstract

    Here we investigated the role of endogenous nitric oxide (NO) and peroxynitrite in the process of protein oxidation (as measured by the detection of 2,4-dinitrophenylhydrazine-reactive carbonyls) in immunostimulated macrophages. Immunostimulation of the macrophages by bacterial lipopolysaccharide and gamma-interferon (LPS/IFNγ) resulted in a marked increase in the oxidation of a large number of mitochondrial and nuclear proteins. The inhibitor of NO synthase, N(G)-methyl-L-arginine (3 mM), and the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis(4-benzoic acid)porphyrin (300 μM) both reduced the extent of protein oxidation in response to LPS/IFNγ. These results support the view that endogenously produced peroxynitrite induces protein oxidation in the mitochondria and nucleus of immunostimulated macrophages.

    Original languageEnglish (US)
    Pages (from-to)147-150
    Number of pages4
    JournalFEBS Letters
    Volume409
    Issue number2
    DOIs
    StatePublished - Jun 9 1997

    Keywords

    • Inflammation
    • Macrophage
    • Nitric oxide
    • Oxidation
    • Peroxynitrite
    • Protein
    • Shock
    • Superoxide

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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