Endogenously produced peroxynitrite induces the oxidation of mitochondrial and nuclear proteins in immunostimulated macrophages

Csaba Szabó, Michael O'Connor, Andrew L. Salzman

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Here we investigated the role of endogenous nitric oxide (NO) and peroxynitrite in the process of protein oxidation (as measured by the detection of 2,4-dinitrophenylhydrazine-reactive carbonyls) in immunostimulated macrophages. Immunostimulation of the macrophages by bacterial lipopolysaccharide and gamma-interferon (LPS/IFNγ) resulted in a marked increase in the oxidation of a large number of mitochondrial and nuclear proteins. The inhibitor of NO synthase, N(G)-methyl-L-arginine (3 mM), and the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis(4-benzoic acid)porphyrin (300 μM) both reduced the extent of protein oxidation in response to LPS/IFNγ. These results support the view that endogenously produced peroxynitrite induces protein oxidation in the mitochondria and nucleus of immunostimulated macrophages.

Original languageEnglish (US)
Pages (from-to)147-150
Number of pages4
JournalFEBS Letters
Volume409
Issue number2
DOIs
StatePublished - Jun 9 1997
Externally publishedYes

Keywords

  • Inflammation
  • Macrophage
  • Nitric oxide
  • Oxidation
  • Peroxynitrite
  • Protein
  • Shock
  • Superoxide

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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