Endothelial cell behaviour on gas-plasma-treated PLA surfaces: The roles of surface chemistry and roughness

Amita Shah, Sarita Shah, Gopinath Mani, Joseph Wenke, Mauli Agrawal

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Glow-discharge gas-plasma (GP) treatment has been shown to induce surface modifications such that cell adhesion and growth are enhanced. However, it is not known which gas used in GP treatment is optimal for endothelial cell function. Polylactic acid (PLA) films treated oxygen, argon, or nitrogen GP were characterized using contact angles, scanning electron microscopy, atomic force microscopy, optical profilometry, and x-ray photoelectron spectroscopy. All three GP treatments decreased the carbon atomic concentration and surface roughness and increased the oxygen atomic concentration. Human umbilical vein endothelial cells were cultured on the PLA films for up to 7 days. Based on proliferation and live/dead assays, surface chemistry was shown to have the greatest effect on the attachment, proliferation, and viability of these cells, while roughness did not have a significant influence. Of the different gases, endothelial cell viability, attachment and proliferation were most significantly increased on PLA surfaces treated with oxygen and argon gas plasma.

Original languageEnglish (US)
Pages (from-to)301-312
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number4
StatePublished - Apr 2011
Externally publishedYes


  • Cell attachment
  • Cell viability
  • Endothelial cells
  • Gas-plasma treatment
  • Polylactic acid
  • Surface characterization

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


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