Endotoxin-induced uveitis in rodents

Umesh C S Yadav, Kota Ramana

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Uveitis is a common cause of vision loss, accounting for 10-15 % of all cases of blindness worldwide and affects individuals of all ages, genders, and races. Uveitis represents a broad range of intraocular inflammatory conditions due to complications of autoimmune diseases, bacterial infections, viral infections, and chemical and metabolic injuries. Endotoxin-induced uveitis (EIU) in rodents is an efficient experimental model to investigate the pathological mechanism and pharmacological efficacy of potential drug agents. EIU is characterized by clinically relevant classical signs of inflammation, including inflammatory exudates and cells in the anterior and vitreous chambers. EIU in small animal models such as rats, mice, and rabbits is a short-lived uveal inflammation that can be developed subsequent to administration of bacterial endotoxin, such as lipopolysaccharide. Here, we present a reproducible, reliable, and simplified protocol to induce EIU in mice. This method could be used with similar efficacy for EIU induction in other small animals as well.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages155-162
Number of pages8
Volume1031
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1031
ISSN (Print)10643745

Fingerprint

Uveitis
Endotoxins
Rodentia
Inflammation
Anterior Chamber
Exudates and Transudates
Virus Diseases
Blindness
Bacterial Infections
Autoimmune Diseases
Lipopolysaccharides
Theoretical Models
Animal Models
Pharmacology
Rabbits
Wounds and Injuries
Pharmaceutical Preparations

Keywords

  • Endotoxin-induced uveitis
  • Eyes
  • Inflammation
  • LPS
  • Mouse

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yadav, U. C. S., & Ramana, K. (2013). Endotoxin-induced uveitis in rodents. In Methods in Molecular Biology (Vol. 1031, pp. 155-162). (Methods in Molecular Biology; Vol. 1031). https://doi.org/10.1007/978-1-62703-481-4_18

Endotoxin-induced uveitis in rodents. / Yadav, Umesh C S; Ramana, Kota.

Methods in Molecular Biology. Vol. 1031 2013. p. 155-162 (Methods in Molecular Biology; Vol. 1031).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yadav, UCS & Ramana, K 2013, Endotoxin-induced uveitis in rodents. in Methods in Molecular Biology. vol. 1031, Methods in Molecular Biology, vol. 1031, pp. 155-162. https://doi.org/10.1007/978-1-62703-481-4_18
Yadav UCS, Ramana K. Endotoxin-induced uveitis in rodents. In Methods in Molecular Biology. Vol. 1031. 2013. p. 155-162. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-481-4_18
Yadav, Umesh C S ; Ramana, Kota. / Endotoxin-induced uveitis in rodents. Methods in Molecular Biology. Vol. 1031 2013. pp. 155-162 (Methods in Molecular Biology).
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