Endotoxin-induced uveitis in rodents

Umesh C.S. Yadav, Kota V. Ramana

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Uveitis is a common cause of vision loss, accounting for 10-15 % of all cases of blindness worldwide and affects individuals of all ages, genders, and races. Uveitis represents a broad range of intraocular inflammatory conditions due to complications of autoimmune diseases, bacterial infections, viral infections, and chemical and metabolic injuries. Endotoxin-induced uveitis (EIU) in rodents is an efficient experimental model to investigate the pathological mechanism and pharmacological efficacy of potential drug agents. EIU is characterized by clinically relevant classical signs of inflammation, including inflammatory exudates and cells in the anterior and vitreous chambers. EIU in small animal models such as rats, mice, and rabbits is a short-lived uveal inflammation that can be developed subsequent to administration of bacterial endotoxin, such as lipopolysaccharide. Here, we present a reproducible, reliable, and simplified protocol to induce EIU in mice. This method could be used with similar efficacy for EIU induction in other small animals as well.

Original languageEnglish (US)
Title of host publicationMouse Models of Innate Immunity
Subtitle of host publicationMethods and Protocols
EditorsIrving C. Allen
Pages155-162
Number of pages8
DOIs
StatePublished - Jul 24 2013

Publication series

NameMethods in Molecular Biology
Volume1031
ISSN (Print)1064-3745

Keywords

  • Endotoxin-induced uveitis
  • Eyes
  • Inflammation
  • LPS
  • Mouse

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Yadav, U. C. S., & Ramana, K. V. (2013). Endotoxin-induced uveitis in rodents. In I. C. Allen (Ed.), Mouse Models of Innate Immunity: Methods and Protocols (pp. 155-162). (Methods in Molecular Biology; Vol. 1031). https://doi.org/10.1007/978-1-62703-481-4_18