Endotoxin-induced uveitis in rodents

Umesh C.S. Yadav, Kota Ramana

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Uveitis can affect individuals of all ages, genders, and races and accounts for 10–15% of all cases of blindness. Uveitis represents a diverse array of intraocular inflammatory conditions that can be associated with complications from autoimmune diseases, bacterial infections, viral infections, chemical injuries, and metabolic issues. In rodents, endotoxin-induced uveitis (EIU) is an efficient experimental model to study pathological mechanisms associated with the disease and evaluate the pharmacological efficacy of potential new drug agents. In the EIU model, uveitis is characterized by clinically relevant inflammation, including inflammatory exudates and cells infiltrated into the anterior and vitreous eye chambers. EIU in small animal models, including rats, mice, and rabbits, is characterized by a short-lived uveal inflammation. This inflammation can be facilitated using bacterial endotoxins, such as lipopolysaccharide (LPS). In this chapter, we present a reliable, reproducible, and simplified protocol to induce EIU in mice. This method is flexible and can be applied for EIU induction in other small animals and rodents.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages161-168
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1960
ISSN (Print)1064-3745

Fingerprint

Uveitis
Endotoxins
Rodentia
Inflammation
Exudates and Transudates
Virus Diseases
Blindness
Bacterial Infections
Autoimmune Diseases
Lipopolysaccharides
Theoretical Models
Animal Models
Pharmacology
Rabbits
Wounds and Injuries
Pharmaceutical Preparations

Keywords

  • Endotoxin-induced uveitis
  • Eyes
  • Inflammation
  • LPS
  • Mice

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yadav, U. C. S., & Ramana, K. (2019). Endotoxin-induced uveitis in rodents. In Methods in Molecular Biology (pp. 161-168). (Methods in Molecular Biology; Vol. 1960). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9167-9_14

Endotoxin-induced uveitis in rodents. / Yadav, Umesh C.S.; Ramana, Kota.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 161-168 (Methods in Molecular Biology; Vol. 1960).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yadav, UCS & Ramana, K 2019, Endotoxin-induced uveitis in rodents. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1960, Humana Press Inc., pp. 161-168. https://doi.org/10.1007/978-1-4939-9167-9_14
Yadav UCS, Ramana K. Endotoxin-induced uveitis in rodents. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 161-168. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9167-9_14
Yadav, Umesh C.S. ; Ramana, Kota. / Endotoxin-induced uveitis in rodents. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 161-168 (Methods in Molecular Biology).
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