Endotoxin priming improves clearance of Pseudomonas aeruginosa in wild-type and interleukin-10 knockout mice

Tushar K. Varma, Megan Durham, Erle D. Murphey, Weihua Cui, Zhiyu Huang, Cheng Y. Lin, Tracy Toliver-Kinsky, Edward R. Sherwood

Research output: Contribution to journalArticle

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Abstract

Endotoxin (lipopolysaccharide [LPS]) tolerance is an altered state of immunity caused by prior exposure to LPS, in which production of many cytokines, including gamma interferon (IFN-γ) and interleukin-12 (IL-12), are reduced but secretion of the anti-inflammatory cytokine IL-10 is increased in response to a subsequent LPS challenge. This pattern of cytokine production is also characteristic of postinflammatory immunosuppression. Therefore, we hypothesized that LPS-primed mice would exhibit an impaired ability to respond to systemic infection with the opportunistic pathogen Pseudomonas aeruginosa. We further hypothesized that depletion of IL-10 would reverse the endotoxin-tolerant state. To test this hypothesis, systemic clearance of Pseudomonas aeruginosa was measured for LPS-primed wild-type and IL-10-deficient mice. LPS-primed wild-type mice exhibited significant suppression of LPS-induced IFN-γ and IL-12 but increased IL-10 production in blood and spleen compared to levels exhibited by saline-primed wild-type mice. The suppressed production of IFN-γ and IL-12 caused by LPS priming was ablated in the spleens, but not blood, of IL-10 knockout mice. LPS-primed wild-type mice cleared Pseudomonas aeruginosa from lungs and blood more effectively than saline-primed mice. LPS-primed IL-10-deficient mice were particularly efficient in clearing Pseudomonas aeruginosa after systemic challenge. These studies show that induction of LPS tolerance enhanced systemic clearance of Pseudomonas aeruginosa and that this effect was augmented by neutralization of IL-10.

Original languageEnglish (US)
Pages (from-to)7340-7347
Number of pages8
JournalInfection and Immunity
Volume73
Issue number11
DOIs
StatePublished - Nov 2005

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Knockout Mice
Endotoxins
Interleukin-10
Pseudomonas aeruginosa
Lipopolysaccharides
Interleukin-12
Cytokines
Interferons
Spleen
Opportunistic Infections
Immunosuppression
Interferon-gamma
Immunity
Anti-Inflammatory Agents
Lung

ASJC Scopus subject areas

  • Immunology

Cite this

Varma, T. K., Durham, M., Murphey, E. D., Cui, W., Huang, Z., Lin, C. Y., ... Sherwood, E. R. (2005). Endotoxin priming improves clearance of Pseudomonas aeruginosa in wild-type and interleukin-10 knockout mice. Infection and Immunity, 73(11), 7340-7347. https://doi.org/10.1128/IAI.73.11.7340-7347.2005

Endotoxin priming improves clearance of Pseudomonas aeruginosa in wild-type and interleukin-10 knockout mice. / Varma, Tushar K.; Durham, Megan; Murphey, Erle D.; Cui, Weihua; Huang, Zhiyu; Lin, Cheng Y.; Toliver-Kinsky, Tracy; Sherwood, Edward R.

In: Infection and Immunity, Vol. 73, No. 11, 11.2005, p. 7340-7347.

Research output: Contribution to journalArticle

Varma, Tushar K. ; Durham, Megan ; Murphey, Erle D. ; Cui, Weihua ; Huang, Zhiyu ; Lin, Cheng Y. ; Toliver-Kinsky, Tracy ; Sherwood, Edward R. / Endotoxin priming improves clearance of Pseudomonas aeruginosa in wild-type and interleukin-10 knockout mice. In: Infection and Immunity. 2005 ; Vol. 73, No. 11. pp. 7340-7347.
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