Abstract
We have developed genetically modified Ae. aegypti mosquitoes that activate the conserved antiviral JAK/STAT pathway in the fat body tissue, by overexpressing either the receptor Dome or the Janus kinase Hop by the blood feeding-induced vitellogenin (Vg) promoter. Transgene expression inhibits infection with several dengue virus (DENV) serotypes in the midgut as well as systemically and in the salivary glands. The impact of the transgenes Dome and Hop on mosquito longevity was minimal, but it resulted in a compromised fecundity when compared to wild-type mosquitoes. Overexpression of Dome and Hop resulted in profound transcriptome regulation in the fat body tissue as well as the midgut tissue, pinpointing several expression signatures that reflect mechanisms of DENV restriction. Our transcriptome studies and reverse genetic analyses suggested that enrichment of DENV restriction factor and depletion of DENV host factor transcripts likely accounts for the DENV inhibition, and they allowed us to identify novel factors that modulate infection. Interestingly, the fat body-specific activation of the JAK/STAT pathway did not result in any enhanced resistance to Zika virus (ZIKV) or chikungunya virus (CHIKV) infection, thereby indicating a possible specialization of the pathway’s antiviral role.
| Original language | English (US) |
|---|---|
| Article number | e0005187 |
| Journal | PLoS Neglected Tropical Diseases |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 12 2017 |
Fingerprint
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- Public Health, Environmental and Occupational Health
- Infectious Diseases
Cite this
Engineered Aedes aegypti JAK/STAT Pathway-Mediated Immunity to Dengue Virus. / Jupatanakul, Natapong; Sim, Shuzhen; Angleró-Rodríguez, Yesseinia I.; Souza-Neto, Jayme; Das, Suchismita; Poti, Kristin E.; Rossi, Shannan; Bergren, Nicholas; Vasilakis, Nikos; Dimopoulos, George.
In: PLoS Neglected Tropical Diseases, Vol. 11, No. 1, e0005187, 12.01.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Engineered Aedes aegypti JAK/STAT Pathway-Mediated Immunity to Dengue Virus
AU - Jupatanakul, Natapong
AU - Sim, Shuzhen
AU - Angleró-Rodríguez, Yesseinia I.
AU - Souza-Neto, Jayme
AU - Das, Suchismita
AU - Poti, Kristin E.
AU - Rossi, Shannan
AU - Bergren, Nicholas
AU - Vasilakis, Nikos
AU - Dimopoulos, George
PY - 2017/1/12
Y1 - 2017/1/12
N2 - We have developed genetically modified Ae. aegypti mosquitoes that activate the conserved antiviral JAK/STAT pathway in the fat body tissue, by overexpressing either the receptor Dome or the Janus kinase Hop by the blood feeding-induced vitellogenin (Vg) promoter. Transgene expression inhibits infection with several dengue virus (DENV) serotypes in the midgut as well as systemically and in the salivary glands. The impact of the transgenes Dome and Hop on mosquito longevity was minimal, but it resulted in a compromised fecundity when compared to wild-type mosquitoes. Overexpression of Dome and Hop resulted in profound transcriptome regulation in the fat body tissue as well as the midgut tissue, pinpointing several expression signatures that reflect mechanisms of DENV restriction. Our transcriptome studies and reverse genetic analyses suggested that enrichment of DENV restriction factor and depletion of DENV host factor transcripts likely accounts for the DENV inhibition, and they allowed us to identify novel factors that modulate infection. Interestingly, the fat body-specific activation of the JAK/STAT pathway did not result in any enhanced resistance to Zika virus (ZIKV) or chikungunya virus (CHIKV) infection, thereby indicating a possible specialization of the pathway’s antiviral role.
AB - We have developed genetically modified Ae. aegypti mosquitoes that activate the conserved antiviral JAK/STAT pathway in the fat body tissue, by overexpressing either the receptor Dome or the Janus kinase Hop by the blood feeding-induced vitellogenin (Vg) promoter. Transgene expression inhibits infection with several dengue virus (DENV) serotypes in the midgut as well as systemically and in the salivary glands. The impact of the transgenes Dome and Hop on mosquito longevity was minimal, but it resulted in a compromised fecundity when compared to wild-type mosquitoes. Overexpression of Dome and Hop resulted in profound transcriptome regulation in the fat body tissue as well as the midgut tissue, pinpointing several expression signatures that reflect mechanisms of DENV restriction. Our transcriptome studies and reverse genetic analyses suggested that enrichment of DENV restriction factor and depletion of DENV host factor transcripts likely accounts for the DENV inhibition, and they allowed us to identify novel factors that modulate infection. Interestingly, the fat body-specific activation of the JAK/STAT pathway did not result in any enhanced resistance to Zika virus (ZIKV) or chikungunya virus (CHIKV) infection, thereby indicating a possible specialization of the pathway’s antiviral role.
UR - http://www.scopus.com/inward/record.url?scp=85012918899&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85012918899&partnerID=8YFLogxK
U2 - 10.1371/journal.pntd.0005187
DO - 10.1371/journal.pntd.0005187
M3 - Article
C2 - 28081143
AN - SCOPUS:85012918899
VL - 11
JO - PLoS Neglected Tropical Diseases
JF - PLoS Neglected Tropical Diseases
SN - 1935-2727
IS - 1
M1 - e0005187
ER -