Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices

Madhubrata Ghosh; Loo Chien Wang; Roland G. Huber; Yunfeng Gao; Leslie K. Morgan; Nikhil Kumar Tulsian; Peter J. Bond; Linda J. Kenney; Ganesh S. Anand

doi:10.1016/j.str.2018.10.012

Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices

Madhubrata Ghosh
, Loo Chien Wang
, Roland G. Huber
, Yunfeng Gao
, Leslie K. Morgan
, Nikhil Kumar Tulsian
, Peter J. Bond
, Linda J. Kenney
, Ganesh S. Anand

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A His-Asp/Glu dyad forms the core of EnvZ histidine kinase (HK) osmosensing wherein osmolytes mediate changes in the backbone and side-chain microenvironment, leading to enhanced histidine autophosphorylation. This His-Asp/Glu dyad is highly conserved across diverse HKs, suggesting a unified model for integrating diverse environmental stimuli to a singular histidine phosphorylation response.

Original language	English (US)
Pages (from-to)	302-314.e4
Journal	Structure
Volume	27
Issue number	2
DOIs	https://doi.org/10.1016/j.str.2018.10.012
State	Published - Feb 5 2019
Externally published	Yes

Keywords

EnvZ
H bonding
His-Asp/Glu dyad
amide hydrogen-deuterium exchange mass spectrometry
helix stabilization
histidine rotamerization
osmosensing
protein dynamics
two-component signal transduction

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/j.str.2018.10.012

Cite this

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title = "Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices",

abstract = "A His-Asp/Glu dyad forms the core of EnvZ histidine kinase (HK) osmosensing wherein osmolytes mediate changes in the backbone and side-chain microenvironment, leading to enhanced histidine autophosphorylation. This His-Asp/Glu dyad is highly conserved across diverse HKs, suggesting a unified model for integrating diverse environmental stimuli to a singular histidine phosphorylation response.",

keywords = "EnvZ, H bonding, His-Asp/Glu dyad, amide hydrogen-deuterium exchange mass spectrometry, helix stabilization, histidine rotamerization, osmosensing, protein dynamics, two-component signal transduction",

author = "Madhubrata Ghosh and Wang, \{Loo Chien\} and Huber, \{Roland G.\} and Yunfeng Gao and Morgan, \{Leslie K.\} and Tulsian, \{Nikhil Kumar\} and Bond, \{Peter J.\} and Kenney, \{Linda J.\} and Anand, \{Ganesh S.\}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2019",

month = feb,

day = "5",

doi = "10.1016/j.str.2018.10.012",

language = "English (US)",

volume = "27",

pages = "302--314.e4",

journal = "Structure",

issn = "0969-2126",

publisher = "Cell Press",

number = "2",

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TY - JOUR

T1 - Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices

AU - Ghosh, Madhubrata

AU - Wang, Loo Chien

AU - Huber, Roland G.

AU - Gao, Yunfeng

AU - Morgan, Leslie K.

AU - Tulsian, Nikhil Kumar

AU - Bond, Peter J.

AU - Kenney, Linda J.

AU - Anand, Ganesh S.

PY - 2019/2/5

Y1 - 2019/2/5

N2 - A His-Asp/Glu dyad forms the core of EnvZ histidine kinase (HK) osmosensing wherein osmolytes mediate changes in the backbone and side-chain microenvironment, leading to enhanced histidine autophosphorylation. This His-Asp/Glu dyad is highly conserved across diverse HKs, suggesting a unified model for integrating diverse environmental stimuli to a singular histidine phosphorylation response.

AB - A His-Asp/Glu dyad forms the core of EnvZ histidine kinase (HK) osmosensing wherein osmolytes mediate changes in the backbone and side-chain microenvironment, leading to enhanced histidine autophosphorylation. This His-Asp/Glu dyad is highly conserved across diverse HKs, suggesting a unified model for integrating diverse environmental stimuli to a singular histidine phosphorylation response.

KW - EnvZ

KW - H bonding

KW - His-Asp/Glu dyad

KW - amide hydrogen-deuterium exchange mass spectrometry

KW - helix stabilization

KW - histidine rotamerization

KW - osmosensing

KW - protein dynamics

KW - two-component signal transduction

UR - https://www.scopus.com/pages/publications/85060705702

UR - https://www.scopus.com/pages/publications/85060705702#tab=citedBy

U2 - 10.1016/j.str.2018.10.012

DO - 10.1016/j.str.2018.10.012

M3 - Article

C2 - 30503779

AN - SCOPUS:85060705702

SN - 0969-2126

VL - 27

SP - 302-314.e4

JO - Structure

JF - Structure

IS - 2

ER -

Engineering an Osmosensor by Pivotal Histidine Positioning within Disordered Helices

Abstract

Keywords

ASJC Scopus subject areas

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