Abstract
Background: IgE-dependent activation of mast cells and basophils through the high-affinity IgE receptor (FcεRI) is involved in the pathogenesis of allergen-induced immediate and late responses. Objective: We investigated the expression and cellular distribution of FcεRI in the nasal mucosa after allergen challenge in patients with summer hay fever. Methods: Fourteen grass pollen-sensitive patients and seven normal control subjects underwent nasal challenge with grass pollen and allergen diluent in random order separated by 2 weeks. Nasal airway caliber was monitored by acoustic rhinometry, and nasal biopsy was performed at 6 hours. Messenger RNA for FcεRI was determined by using reverse-transcription polymerase chain reaction, and FcεRI protein expression was determined by immunohistology with a mouse monoclonal antibody (22E7) and a rabbit polyclonal antibody (997) directed against the α subunit. Co-localization of FcεRI receptors was performed by using double- immunostaining methods. Results: In atopic subjects, there was a significant early decrease in nasal airway caliber, which extended up to 6 hours after allergen challenge. FcεRI mRNA levels were elevated at 6 hours (p = 0.03). Cells expressing FcεRI protein were increased in patients with atopic rhinitis compared with normal control subjects (p = 0.03). Further increases in FcεRI+ cells were observed after allergen challenge only in the atopic group (p = 0.02). Double immunohistochemistry revealed that the majority of FcεRI+ cells were mast cells (64%), followed by macrophages (20%), eosinophils (4%), and dendritic cells (2%), with 10% FcεRI+ cells being unidentified. Conclusions: Our results demonstrate increased FcεRI expression during allergen-induced rhinitis and highlight a potential target for treatment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 78-86 |
| Number of pages | 9 |
| Journal | Journal of Allergy and Clinical Immunology |
| Volume | 100 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1997 |
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Keywords
- Allergic rhinitis
- FcεRI receptors
- IgE
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Enhanced expression of high-affinity IgE receptor (FcεRI) α chain in human allergen-induced rhinitis with co-localization to mast cells, macrophages, eosinophils, and dendritic cells. / Rajakulasingam, Karalasingam; Durham, Stephen R.; O'Brien, Fiona; Humbert, Mark; Barata, Luis T.; Reece, Lisa; Kay, A. Barry; Grant, J. Andrew.
In: Journal of Allergy and Clinical Immunology, Vol. 100, No. 1, 1997, p. 78-86.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced expression of high-affinity IgE receptor (FcεRI) α chain in human allergen-induced rhinitis with co-localization to mast cells, macrophages, eosinophils, and dendritic cells
AU - Rajakulasingam, Karalasingam
AU - Durham, Stephen R.
AU - O'Brien, Fiona
AU - Humbert, Mark
AU - Barata, Luis T.
AU - Reece, Lisa
AU - Kay, A. Barry
AU - Grant, J. Andrew
PY - 1997
Y1 - 1997
N2 - Background: IgE-dependent activation of mast cells and basophils through the high-affinity IgE receptor (FcεRI) is involved in the pathogenesis of allergen-induced immediate and late responses. Objective: We investigated the expression and cellular distribution of FcεRI in the nasal mucosa after allergen challenge in patients with summer hay fever. Methods: Fourteen grass pollen-sensitive patients and seven normal control subjects underwent nasal challenge with grass pollen and allergen diluent in random order separated by 2 weeks. Nasal airway caliber was monitored by acoustic rhinometry, and nasal biopsy was performed at 6 hours. Messenger RNA for FcεRI was determined by using reverse-transcription polymerase chain reaction, and FcεRI protein expression was determined by immunohistology with a mouse monoclonal antibody (22E7) and a rabbit polyclonal antibody (997) directed against the α subunit. Co-localization of FcεRI receptors was performed by using double- immunostaining methods. Results: In atopic subjects, there was a significant early decrease in nasal airway caliber, which extended up to 6 hours after allergen challenge. FcεRI mRNA levels were elevated at 6 hours (p = 0.03). Cells expressing FcεRI protein were increased in patients with atopic rhinitis compared with normal control subjects (p = 0.03). Further increases in FcεRI+ cells were observed after allergen challenge only in the atopic group (p = 0.02). Double immunohistochemistry revealed that the majority of FcεRI+ cells were mast cells (64%), followed by macrophages (20%), eosinophils (4%), and dendritic cells (2%), with 10% FcεRI+ cells being unidentified. Conclusions: Our results demonstrate increased FcεRI expression during allergen-induced rhinitis and highlight a potential target for treatment.
AB - Background: IgE-dependent activation of mast cells and basophils through the high-affinity IgE receptor (FcεRI) is involved in the pathogenesis of allergen-induced immediate and late responses. Objective: We investigated the expression and cellular distribution of FcεRI in the nasal mucosa after allergen challenge in patients with summer hay fever. Methods: Fourteen grass pollen-sensitive patients and seven normal control subjects underwent nasal challenge with grass pollen and allergen diluent in random order separated by 2 weeks. Nasal airway caliber was monitored by acoustic rhinometry, and nasal biopsy was performed at 6 hours. Messenger RNA for FcεRI was determined by using reverse-transcription polymerase chain reaction, and FcεRI protein expression was determined by immunohistology with a mouse monoclonal antibody (22E7) and a rabbit polyclonal antibody (997) directed against the α subunit. Co-localization of FcεRI receptors was performed by using double- immunostaining methods. Results: In atopic subjects, there was a significant early decrease in nasal airway caliber, which extended up to 6 hours after allergen challenge. FcεRI mRNA levels were elevated at 6 hours (p = 0.03). Cells expressing FcεRI protein were increased in patients with atopic rhinitis compared with normal control subjects (p = 0.03). Further increases in FcεRI+ cells were observed after allergen challenge only in the atopic group (p = 0.02). Double immunohistochemistry revealed that the majority of FcεRI+ cells were mast cells (64%), followed by macrophages (20%), eosinophils (4%), and dendritic cells (2%), with 10% FcεRI+ cells being unidentified. Conclusions: Our results demonstrate increased FcεRI expression during allergen-induced rhinitis and highlight a potential target for treatment.
KW - Allergic rhinitis
KW - FcεRI receptors
KW - IgE
UR - http://www.scopus.com/inward/record.url?scp=0030837924&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030837924&partnerID=8YFLogxK
U2 - 10.1016/S0091-6749(97)70198-2
DO - 10.1016/S0091-6749(97)70198-2
M3 - Article
C2 - 9257791
AN - SCOPUS:0030837924
VL - 100
SP - 78
EP - 86
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 1
ER -