Enhanced myocardial function in transgenic mice overexpressing the β2-adrenergic receptor

C. A. Milano, L. F. Allen, H. A. Rockman, P. C. Dolber, T. R. McMinn, K. R. Chien, T. D. Johnson, R. A. Bond, R. J. Lefkowitz

Research output: Contribution to journalArticle

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Abstract

Transgenic mice were created with cardiac-specific overexpression of the β2-adrenergic receptor. This resulted in increased basal myocardial adenylyl cyclase activity, enhanced atrial contractility, and increased left ventricular function in vivo; these parameters at baseline in the transgenic animals were equal to those observed in control animals maximally stimulated with isoproterenol. These results illustrate a useful approach for studying the effect of gene expression on cardiac contractility. Because chronic heart failure in humans is accompanied by a reduction in the number of myocardial β-adrenergic receptors and in inotropic responsiveness, these results suggest a potential gene therapy approach to this disease state.

Original languageEnglish (US)
Pages (from-to)582-586
Number of pages5
JournalScience
Volume264
Issue number5158
StatePublished - Apr 22 1994
Externally publishedYes

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Adrenergic Receptors
Transgenic Mice
Genetically Modified Animals
Left Ventricular Function
Isoproterenol
Adenylyl Cyclases
Genetic Therapy
Heart Failure
Gene Expression

ASJC Scopus subject areas

  • General

Cite this

Milano, C. A., Allen, L. F., Rockman, H. A., Dolber, P. C., McMinn, T. R., Chien, K. R., ... Lefkowitz, R. J. (1994). Enhanced myocardial function in transgenic mice overexpressing the β2-adrenergic receptor. Science, 264(5158), 582-586.

Enhanced myocardial function in transgenic mice overexpressing the β2-adrenergic receptor. / Milano, C. A.; Allen, L. F.; Rockman, H. A.; Dolber, P. C.; McMinn, T. R.; Chien, K. R.; Johnson, T. D.; Bond, R. A.; Lefkowitz, R. J.

In: Science, Vol. 264, No. 5158, 22.04.1994, p. 582-586.

Research output: Contribution to journalArticle

Milano, CA, Allen, LF, Rockman, HA, Dolber, PC, McMinn, TR, Chien, KR, Johnson, TD, Bond, RA & Lefkowitz, RJ 1994, 'Enhanced myocardial function in transgenic mice overexpressing the β2-adrenergic receptor', Science, vol. 264, no. 5158, pp. 582-586.
Milano CA, Allen LF, Rockman HA, Dolber PC, McMinn TR, Chien KR et al. Enhanced myocardial function in transgenic mice overexpressing the β2-adrenergic receptor. Science. 1994 Apr 22;264(5158):582-586.
Milano, C. A. ; Allen, L. F. ; Rockman, H. A. ; Dolber, P. C. ; McMinn, T. R. ; Chien, K. R. ; Johnson, T. D. ; Bond, R. A. ; Lefkowitz, R. J. / Enhanced myocardial function in transgenic mice overexpressing the β2-adrenergic receptor. In: Science. 1994 ; Vol. 264, No. 5158. pp. 582-586.
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