Enhanced nitrosative stress during Trypanosoma cruzi infection causes nitrotyrosine modification of host proteins

Implications in Chagas' disease

Monisha Dhiman, Ernesto Satoshi Nakayasu, Yashoda Hosakote Madaiah, Brobey K. Reynolds, Jian Jun Wen, Igor Correia Almeida, Nisha Garg

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Oxidative/nitrosative stress may be important in the pathology of Chagas' disease. Experimental animals infected by Trypanosoma cruzi showed an early rise in myocardial and peripheral protein-3-nitrotyrosine (3NT) and protein-carbonyl formation that persisted during the chronic stage of disease. In comparison, experimental chronic ethanol-induced cardiomyopathy was slow to develop and presented with a moderate increase in oxidative stress and minimal to no nitrosative stress after long-term alcohol feeding of animals. The oxidative stress in both chagasic animals and animals with ethanol-induced cardiomyopathy correlated with the persistence of reactive oxygen species-producing inflammatory intermediates. Protein-3NT formation in T. cruzi-infected animals was associated with enhanced nitric oxide expression (inferred by nitrite/nitrate levels) and myeloperoxidase activity, suggesting that both peroxynitrite- and myeloperoxidase-mediated pathways contribute to increased protein nitration in Chagas' disease. We used one- and two-dimensional gel electrophoresis and Western blot analysis to identify disease-specific plasma proteins that were 3NT-modified in T. cruzi-infected animals. Nitrated protein spots (56 in total) were sequenced by matrix-assisted laser desorption ionization/time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry and identified by a homology search of public databases. Clustering of 3NT-modified proteins according to their functional characteristics revealed that the nitration of immunoglobulins, apolipoprotein isoforms, and other proteins might perturb their functions and be important in the pathology of Chagas' disease. We also showed that nitrated peptides derived from titin and α-actin were released into the plasma of patients with Chagas' disease. Such modified proteins may be useful biomarkers of Chagas' disease.

Original languageEnglish (US)
Pages (from-to)728-740
Number of pages13
JournalAmerican Journal of Pathology
Volume173
Issue number3
DOIs
StatePublished - Sep 2008

Fingerprint

Chagas Disease
Trypanosoma cruzi
Infection
Proteins
Oxidative Stress
Cardiomyopathies
Peroxidase
Ethanol
Protein Carbonylation
Connectin
Pathology
Peroxynitrous Acid
Apolipoproteins
Electrophoresis, Gel, Two-Dimensional
Nitrites
Tandem Mass Spectrometry
Liquid Chromatography
Nitrates
Cluster Analysis
3-nitrotyrosine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Enhanced nitrosative stress during Trypanosoma cruzi infection causes nitrotyrosine modification of host proteins : Implications in Chagas' disease. / Dhiman, Monisha; Nakayasu, Ernesto Satoshi; Hosakote Madaiah, Yashoda; Reynolds, Brobey K.; Wen, Jian Jun; Almeida, Igor Correia; Garg, Nisha.

In: American Journal of Pathology, Vol. 173, No. 3, 09.2008, p. 728-740.

Research output: Contribution to journalArticle

Dhiman, Monisha ; Nakayasu, Ernesto Satoshi ; Hosakote Madaiah, Yashoda ; Reynolds, Brobey K. ; Wen, Jian Jun ; Almeida, Igor Correia ; Garg, Nisha. / Enhanced nitrosative stress during Trypanosoma cruzi infection causes nitrotyrosine modification of host proteins : Implications in Chagas' disease. In: American Journal of Pathology. 2008 ; Vol. 173, No. 3. pp. 728-740.
@article{0fc577a0605f4ff09567432713618a7d,
title = "Enhanced nitrosative stress during Trypanosoma cruzi infection causes nitrotyrosine modification of host proteins: Implications in Chagas' disease",
abstract = "Oxidative/nitrosative stress may be important in the pathology of Chagas' disease. Experimental animals infected by Trypanosoma cruzi showed an early rise in myocardial and peripheral protein-3-nitrotyrosine (3NT) and protein-carbonyl formation that persisted during the chronic stage of disease. In comparison, experimental chronic ethanol-induced cardiomyopathy was slow to develop and presented with a moderate increase in oxidative stress and minimal to no nitrosative stress after long-term alcohol feeding of animals. The oxidative stress in both chagasic animals and animals with ethanol-induced cardiomyopathy correlated with the persistence of reactive oxygen species-producing inflammatory intermediates. Protein-3NT formation in T. cruzi-infected animals was associated with enhanced nitric oxide expression (inferred by nitrite/nitrate levels) and myeloperoxidase activity, suggesting that both peroxynitrite- and myeloperoxidase-mediated pathways contribute to increased protein nitration in Chagas' disease. We used one- and two-dimensional gel electrophoresis and Western blot analysis to identify disease-specific plasma proteins that were 3NT-modified in T. cruzi-infected animals. Nitrated protein spots (56 in total) were sequenced by matrix-assisted laser desorption ionization/time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry and identified by a homology search of public databases. Clustering of 3NT-modified proteins according to their functional characteristics revealed that the nitration of immunoglobulins, apolipoprotein isoforms, and other proteins might perturb their functions and be important in the pathology of Chagas' disease. We also showed that nitrated peptides derived from titin and α-actin were released into the plasma of patients with Chagas' disease. Such modified proteins may be useful biomarkers of Chagas' disease.",
author = "Monisha Dhiman and Nakayasu, {Ernesto Satoshi} and {Hosakote Madaiah}, Yashoda and Reynolds, {Brobey K.} and Wen, {Jian Jun} and Almeida, {Igor Correia} and Nisha Garg",
year = "2008",
month = "9",
doi = "10.2353/ajpath.2008.080047",
language = "English (US)",
volume = "173",
pages = "728--740",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Enhanced nitrosative stress during Trypanosoma cruzi infection causes nitrotyrosine modification of host proteins

T2 - Implications in Chagas' disease

AU - Dhiman, Monisha

AU - Nakayasu, Ernesto Satoshi

AU - Hosakote Madaiah, Yashoda

AU - Reynolds, Brobey K.

AU - Wen, Jian Jun

AU - Almeida, Igor Correia

AU - Garg, Nisha

PY - 2008/9

Y1 - 2008/9

N2 - Oxidative/nitrosative stress may be important in the pathology of Chagas' disease. Experimental animals infected by Trypanosoma cruzi showed an early rise in myocardial and peripheral protein-3-nitrotyrosine (3NT) and protein-carbonyl formation that persisted during the chronic stage of disease. In comparison, experimental chronic ethanol-induced cardiomyopathy was slow to develop and presented with a moderate increase in oxidative stress and minimal to no nitrosative stress after long-term alcohol feeding of animals. The oxidative stress in both chagasic animals and animals with ethanol-induced cardiomyopathy correlated with the persistence of reactive oxygen species-producing inflammatory intermediates. Protein-3NT formation in T. cruzi-infected animals was associated with enhanced nitric oxide expression (inferred by nitrite/nitrate levels) and myeloperoxidase activity, suggesting that both peroxynitrite- and myeloperoxidase-mediated pathways contribute to increased protein nitration in Chagas' disease. We used one- and two-dimensional gel electrophoresis and Western blot analysis to identify disease-specific plasma proteins that were 3NT-modified in T. cruzi-infected animals. Nitrated protein spots (56 in total) were sequenced by matrix-assisted laser desorption ionization/time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry and identified by a homology search of public databases. Clustering of 3NT-modified proteins according to their functional characteristics revealed that the nitration of immunoglobulins, apolipoprotein isoforms, and other proteins might perturb their functions and be important in the pathology of Chagas' disease. We also showed that nitrated peptides derived from titin and α-actin were released into the plasma of patients with Chagas' disease. Such modified proteins may be useful biomarkers of Chagas' disease.

AB - Oxidative/nitrosative stress may be important in the pathology of Chagas' disease. Experimental animals infected by Trypanosoma cruzi showed an early rise in myocardial and peripheral protein-3-nitrotyrosine (3NT) and protein-carbonyl formation that persisted during the chronic stage of disease. In comparison, experimental chronic ethanol-induced cardiomyopathy was slow to develop and presented with a moderate increase in oxidative stress and minimal to no nitrosative stress after long-term alcohol feeding of animals. The oxidative stress in both chagasic animals and animals with ethanol-induced cardiomyopathy correlated with the persistence of reactive oxygen species-producing inflammatory intermediates. Protein-3NT formation in T. cruzi-infected animals was associated with enhanced nitric oxide expression (inferred by nitrite/nitrate levels) and myeloperoxidase activity, suggesting that both peroxynitrite- and myeloperoxidase-mediated pathways contribute to increased protein nitration in Chagas' disease. We used one- and two-dimensional gel electrophoresis and Western blot analysis to identify disease-specific plasma proteins that were 3NT-modified in T. cruzi-infected animals. Nitrated protein spots (56 in total) were sequenced by matrix-assisted laser desorption ionization/time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry and identified by a homology search of public databases. Clustering of 3NT-modified proteins according to their functional characteristics revealed that the nitration of immunoglobulins, apolipoprotein isoforms, and other proteins might perturb their functions and be important in the pathology of Chagas' disease. We also showed that nitrated peptides derived from titin and α-actin were released into the plasma of patients with Chagas' disease. Such modified proteins may be useful biomarkers of Chagas' disease.

UR - http://www.scopus.com/inward/record.url?scp=51349110109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51349110109&partnerID=8YFLogxK

U2 - 10.2353/ajpath.2008.080047

DO - 10.2353/ajpath.2008.080047

M3 - Article

VL - 173

SP - 728

EP - 740

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 3

ER -