Enhanced superoxide production by alveolar macrophages and air-space cells, airway inflammation, and alveolar macrophage density changes after segmental antigen bronchoprovocation in allergic subjects

William Calhoun, H. E. Reed, D. R. Moest, C. A. Stevens

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

Airway inflammation is a principal determinant of airway responsiveness and function in asthma and allergic diseases. Alveolar macrophages (AM) may contribute to inflammation in multiple ways, including release of reactive oxygen species such as superoxide (SO) anion. We hypothesized that SO production by AM increases after segmental bronchoprovocation (SBP) with relevant antigen and contributes to airway injury. Eight ragweed-sensitive subjects with allergic rhinitis were studied by bronchoalveolar lavage and ragweed SBP to determine the SO production and characteristics of cells recruited after antigen challenge. No significant changes in cell numbers or total protein concentration were observed immediately after antigen challenge. Purification (to > 94%) of AM on discontinuous gradients of Percoll revealed significantly increased spontaneous and opsonized-zymosan- driven SO production immediately after antigen challenge. Forty-eight hours later, total air-space cells, AM, eosinophils, and total protein concentration were significantly increased in relationship to antigen dose given. Furthermore, both unfractionated air-space cells and purified AM obtained 48 h after antigen challenge released increased amounts of SO anion in response to activator compared with either cells obtained immediately after SBP or those obtained 48 h after saline challenge. In addition, significant increases in high density AM were also seen 48 h after antigen challenge. These data suggest that AM activation occurs immediately after antigen challenge, and that the late airway response to antigen is characterized by the appearance of high density AM, which have potentiated SO release. The increased oxidative burden thereby produced may contribute to increased airway injury.

Original languageEnglish (US)
Pages (from-to)317-325
Number of pages9
JournalAmerican Review of Respiratory Disease
Volume145
Issue number2 II SUPPL.
StatePublished - 1992
Externally publishedYes

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Alveolar Macrophages
Superoxides
Air
Inflammation
Antigens
Ambrosia
Zymosan
Macrophage Activation
Wounds and Injuries
Bronchoalveolar Lavage
Eosinophils
Reactive Oxygen Species
Proteins
Asthma
Cell Count

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

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title = "Enhanced superoxide production by alveolar macrophages and air-space cells, airway inflammation, and alveolar macrophage density changes after segmental antigen bronchoprovocation in allergic subjects",
abstract = "Airway inflammation is a principal determinant of airway responsiveness and function in asthma and allergic diseases. Alveolar macrophages (AM) may contribute to inflammation in multiple ways, including release of reactive oxygen species such as superoxide (SO) anion. We hypothesized that SO production by AM increases after segmental bronchoprovocation (SBP) with relevant antigen and contributes to airway injury. Eight ragweed-sensitive subjects with allergic rhinitis were studied by bronchoalveolar lavage and ragweed SBP to determine the SO production and characteristics of cells recruited after antigen challenge. No significant changes in cell numbers or total protein concentration were observed immediately after antigen challenge. Purification (to > 94{\%}) of AM on discontinuous gradients of Percoll revealed significantly increased spontaneous and opsonized-zymosan- driven SO production immediately after antigen challenge. Forty-eight hours later, total air-space cells, AM, eosinophils, and total protein concentration were significantly increased in relationship to antigen dose given. Furthermore, both unfractionated air-space cells and purified AM obtained 48 h after antigen challenge released increased amounts of SO anion in response to activator compared with either cells obtained immediately after SBP or those obtained 48 h after saline challenge. In addition, significant increases in high density AM were also seen 48 h after antigen challenge. These data suggest that AM activation occurs immediately after antigen challenge, and that the late airway response to antigen is characterized by the appearance of high density AM, which have potentiated SO release. The increased oxidative burden thereby produced may contribute to increased airway injury.",
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T1 - Enhanced superoxide production by alveolar macrophages and air-space cells, airway inflammation, and alveolar macrophage density changes after segmental antigen bronchoprovocation in allergic subjects

AU - Calhoun, William

AU - Reed, H. E.

AU - Moest, D. R.

AU - Stevens, C. A.

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