Enhancement of drug delivery in tumors by using interaction of nanoparticles with ultrasound radiation

Irina V. Larina, B. Mark Evers, Taras V. Ashitkov, Christian Bartels, Kirill V. Larin, Rinat Esenaliev

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Efficacy and safety of cancer chemo- and biotherapy are limited by poor penetration of anti-cancer drugs from blood into tumor cells. Tumor blood vessel wall, slow diffusion in the interstitium, and cancer cell membrane create physiological barriers for anti-cancer drugs, in particular promising macromolecular agents. Recently, we proposed to use selective accumulation of exogenous nano- and microparticles in tumors followed by ultrasound-induced cavitation for safe and efficient drug and gene delivery. In this paper, we first investigated the influence of polystyrene nanoparticles (100 and 280 nm in diameter and concentration up to 0.2% w/w) on cavitation threshold in water at the frequency of 20 kHz. Then, using optimal irradiation parameters found in the first part of this work, we studied efficacy of cancer chemotherapy with this technique. The experiments were performed in athymic nude mice bearing human colon KM20 tumors, which are highly resistant to chemotherapy. Ultrasound with the frequency of 20 kHz in combination with i.v. injected polystyrene nanoparticles was applied to enhance delivery of chemotherapeutic agent 5-fluorouracil. Our studies demonstrated that ultrasound irradiation in combination with the nanoparticle and drug injections significantly decreased tumor volume and resulted in complete tumor regression at optimal irradiation conditions, while the volume of control (non-irradiated) tumors increased despite drug injections. These data suggest that ultrasound-induced drug delivery may improve efficacy of current cancer treatment regimens.

Original languageEnglish (US)
Pages (from-to)217-226
Number of pages10
JournalTechnology in Cancer Research and Treatment
Volume4
Issue number2
StatePublished - Apr 2005

Fingerprint

Nanoparticles
Pharmaceutical Preparations
Neoplasms
Polystyrenes
Nude Mice
Drug Therapy
Ultrasonic Waves
Vascular Tissue Neoplasms
Biological Therapy
Injections
Tumor Burden
Fluorouracil
Colon
Cell Membrane
Safety
Water

Keywords

  • Biotherapy
  • Cancer
  • Cavitation
  • Chemotherapy
  • Drug delivery
  • Ultrasound

ASJC Scopus subject areas

  • Cancer Research
  • Radiology Nuclear Medicine and imaging

Cite this

Enhancement of drug delivery in tumors by using interaction of nanoparticles with ultrasound radiation. / Larina, Irina V.; Evers, B. Mark; Ashitkov, Taras V.; Bartels, Christian; Larin, Kirill V.; Esenaliev, Rinat.

In: Technology in Cancer Research and Treatment, Vol. 4, No. 2, 04.2005, p. 217-226.

Research output: Contribution to journalArticle

Larina, Irina V. ; Evers, B. Mark ; Ashitkov, Taras V. ; Bartels, Christian ; Larin, Kirill V. ; Esenaliev, Rinat. / Enhancement of drug delivery in tumors by using interaction of nanoparticles with ultrasound radiation. In: Technology in Cancer Research and Treatment. 2005 ; Vol. 4, No. 2. pp. 217-226.
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