Enhancement of lung tumor colony formation by treatment of mice with monoclonal antibodies to pulmonary capillary endothelial cells

S. J. Kennel, T. K. Lankford, R. L. Ullrich, R. J. Jamasbi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Two rat monoclonal antibodies, 34A and 201B, have been isolated and shown to bind preferentially to capillary endothelial cells in the lung. Administration of these antibodies to mice increases the number of lung colonies derived from i.v. injection of tumor cells. The antibodies increase lung colonization in C57BL/6 mice following i.v. injection of B16-F10 melanoma cells and in BALB/c mice following injection of line 1 lung carcinoma cells. Neither 34A nor 201B monoclonal antibody binds to B16 melanoma or line 1 carcinoma and so must exert its effect by interaction with endothelial cells. Antibodies injected i.v., s.c., or i.p. are active from 1 h to 1 wk if injected before cell injection. The effect is optimal when 0.1 ml of ascites fluid containing 120 μg of antigen binding capacity of both MoAbs 34A and 201B is injected. Significant damage to endothelial cells could not be documented by histopathological examination at the light microscope level or by protein leakage into the air space as measured by lung lavage. However, electron micrographs taken 3 h after monoclonal antibody injection showed minor damage to endothelial cell membranes throughout the lung with some areas of mild edema. The increased colonization may be mediated by this subtle damage to endothelial cells, or antibody interactions with endothelial cells may trigger secondary reactions such as altered expression of growth factors.

Original languageEnglish (US)
Pages (from-to)4964-4968
Number of pages5
JournalCancer Research
Volume48
Issue number17
StatePublished - 1988
Externally publishedYes

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Endothelial Cells
Monoclonal Antibodies
Lung
Injections
Neoplasms
Experimental Melanomas
Antibodies
Therapeutics
Carcinoma
Bronchoalveolar Lavage
Inbred C57BL Mouse
Ascites
Edema
Intercellular Signaling Peptides and Proteins
Air
Cell Membrane
Electrons
Light
Antigens
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Enhancement of lung tumor colony formation by treatment of mice with monoclonal antibodies to pulmonary capillary endothelial cells. / Kennel, S. J.; Lankford, T. K.; Ullrich, R. L.; Jamasbi, R. J.

In: Cancer Research, Vol. 48, No. 17, 1988, p. 4964-4968.

Research output: Contribution to journalArticle

Kennel, S. J. ; Lankford, T. K. ; Ullrich, R. L. ; Jamasbi, R. J. / Enhancement of lung tumor colony formation by treatment of mice with monoclonal antibodies to pulmonary capillary endothelial cells. In: Cancer Research. 1988 ; Vol. 48, No. 17. pp. 4964-4968.
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