Enhancing the utility of a prM/E-expressing chimeric vaccine for Japanese encephalitis by addition of the JEV NS1 gene

Tomohiro Ishikawa, Gongbo Wang, Douglas G. Widman, Ernesto Infante, Evandro R. Winkelmann, Nigel Bourne, Peter W. Mason

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Recently, we demonstrated that a single-cycle West Nile virus (WNV) named RepliVAX WN could be used to produce a chimeric Japanese encephalitis (JE) vaccine (RepliVAX JE) by replacing the WNV prM/E genes with those of JEV. Here, we tested if replacement of WNV NS1 gene in RepliVAX JE with that of JEV (producing TripliVAX JE) could produce a superior vaccine. TripliVAX JE elicited higher anti-E immunity and displayed better efficacy in mice than RepliVAX JE. Furthermore, TripliVAX JE displayed reduced immune interference caused by pre-existing anti-NS1 immunity. Thus, we propose prM/E/NS1 chimerization as a new strategy for flavivirus vaccine development.

Original languageEnglish (US)
Pages (from-to)7444-7455
Number of pages12
JournalVaccine
Volume29
Issue number43
DOIs
StatePublished - Oct 6 2011

Keywords

  • Chimera
  • Japanese encephalitis
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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