Enhancing the utility of a prM/E-expressing chimeric vaccine for Japanese encephalitis by addition of the JEV NS1 gene

Tomohiro Ishikawa; Gongbo Wang; Douglas G. Widman; Ernesto Infante; Evandro R. Winkelmann; Nigel Bourne; Peter W. Mason

doi:10.1016/j.vaccine.2011.07.058

Enhancing the utility of a prM/E-expressing chimeric vaccine for Japanese encephalitis by addition of the JEV NS1 gene

Tomohiro Ishikawa, Gongbo Wang, Douglas G. Widman, Ernesto Infante, Evandro R. Winkelmann, Nigel Bourne, Peter W. Mason

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Recently, we demonstrated that a single-cycle West Nile virus (WNV) named RepliVAX WN could be used to produce a chimeric Japanese encephalitis (JE) vaccine (RepliVAX JE) by replacing the WNV prM/E genes with those of JEV. Here, we tested if replacement of WNV NS1 gene in RepliVAX JE with that of JEV (producing TripliVAX JE) could produce a superior vaccine. TripliVAX JE elicited higher anti-E immunity and displayed better efficacy in mice than RepliVAX JE. Furthermore, TripliVAX JE displayed reduced immune interference caused by pre-existing anti-NS1 immunity. Thus, we propose prM/E/NS1 chimerization as a new strategy for flavivirus vaccine development.

Original language	English (US)
Pages (from-to)	7444-7455
Number of pages	12
Journal	Vaccine
Volume	29
Issue number	43
DOIs	https://doi.org/10.1016/j.vaccine.2011.07.058
State	Published - Oct 6 2011

Keywords

Chimera
Japanese encephalitis
Vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2011.07.058

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title = "Enhancing the utility of a prM/E-expressing chimeric vaccine for Japanese encephalitis by addition of the JEV NS1 gene",

abstract = "Recently, we demonstrated that a single-cycle West Nile virus (WNV) named RepliVAX WN could be used to produce a chimeric Japanese encephalitis (JE) vaccine (RepliVAX JE) by replacing the WNV prM/E genes with those of JEV. Here, we tested if replacement of WNV NS1 gene in RepliVAX JE with that of JEV (producing TripliVAX JE) could produce a superior vaccine. TripliVAX JE elicited higher anti-E immunity and displayed better efficacy in mice than RepliVAX JE. Furthermore, TripliVAX JE displayed reduced immune interference caused by pre-existing anti-NS1 immunity. Thus, we propose prM/E/NS1 chimerization as a new strategy for flavivirus vaccine development.",

keywords = "Chimera, Japanese encephalitis, Vaccine",

author = "Tomohiro Ishikawa and Gongbo Wang and Widman, {Douglas G.} and Ernesto Infante and Winkelmann, {Evandro R.} and Nigel Bourne and Mason, {Peter W.}",

note = "Funding Information: We thank R.B. Tesh (UTMB) for the JEV Beijing P3 strain and WNV NY99 strain. This work was supported by a grant from NIAID to PWM through the Western Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research (NIH grant number U54 AI057156 ), NIH R21 ( AI077077 ), and U01 AI082960-01. DGW was supported for a portion of this work by a James W. McLaughlin fellowship .",

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AU - Infante, Ernesto

AU - Winkelmann, Evandro R.

AU - Bourne, Nigel

AU - Mason, Peter W.

N1 - Funding Information: We thank R.B. Tesh (UTMB) for the JEV Beijing P3 strain and WNV NY99 strain. This work was supported by a grant from NIAID to PWM through the Western Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research (NIH grant number U54 AI057156 ), NIH R21 ( AI077077 ), and U01 AI082960-01. DGW was supported for a portion of this work by a James W. McLaughlin fellowship .

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Enhancing the utility of a prM/E-expressing chimeric vaccine for Japanese encephalitis by addition of the JEV NS1 gene

Abstract

Keywords

ASJC Scopus subject areas

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