Enteral Feeding Preserves Mucosal Immunity Despite in Vivo MAdCAM-1 Blockade of Lymphocyte Homing

Shigeo Ikeda, Kenneth A. Kudsk, Kazuhiko Fukatsu, Cheryl D. Johnson, Tho Le, Shannon Reese, Ben L. Zarzaur, R. Neal Garrison, David Herndon, George M. Watkins, Timothy L. Pruett

Research output: Contribution to journalArticle

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Abstract

Objective: To determine the influence of route of nutrition on gut mucosal addressin cellular adhesion molecule-1 (MAdCAM-1) expression and the effect of MAdCAM-1 blockade on gut-associated lymphoid tissue (GALT) lymphocyte populations and established respiratory antibacterial immunity. Summary: Background Data Lymphocytes, sensitized to antigens in Peyer's patches, migrate via mesenteric lymph nodes and home to intestinal lamina propria. MAdCAM-1 located on endothelial cells regulates this trafficking. Experimentally, parenteral nutrition (PN) decreases GALT cell mass and mucosal immunity when compared with enteral feeding. Methods: In experiment 1, MAdCAM-1 expression was quantified in 32 mice after 4 days of feeding chow, a complex diet, intragastric (IG)-PN, or PN. In experiment 2, MAdCAM-1 was measured in 102 mice 0, 4, 8, 12, 24, 48, or 72 hours after starting PN and at 0, 4, 8, 12, 24, or 48 hours after reinstituting chow following 5 days of PN. In experiment 3, 56 mice received chow, PN, chow + MECA-367 (anti-MAdCAM-1 mAb), or chow + Isotype control Ab (IsoAb) for 5 days, followed by Peyer's patches, lamina propria, and intraepithelial lymphocyte yield with respiratory and intestinal IgA levels. In experiment 4, 10 days after Pseudomonas immunization, mice received chow + MECA-367 or chow + IsoAb for 4 days followed by 1.2 × 108 Pseudomonas intratracheally. Results: Diet and route affect MAdCAM-1 expression (chow > complex diet > IG-PN > PN). Decreased MAdCAM-1 expression occurred within hours of starting PN in Peyer's patches, but not mesenteric lymph nodes or the intestine, and recovered quickly with enteral refeeding. MAdCAM-1 blockade reduced all GALT populations. Blockade had little effect on IgA levels and partially impaired the late response of established respiratory immunity. Conclusions: Enteral feeding affects MAdCAM-1 expression. Complete MAdCAM-1 blockade reduces GALT lymphocytes to PN levels, but the chow feeding stimulus preserves IgA and early antibacterial resistance, implying the existence of non-MAdCAM-1 mechanisms to preserve mucosal immunity.

Original languageEnglish (US)
Pages (from-to)677-685
Number of pages9
JournalAnnals of Surgery
Volume237
Issue number5
DOIs
StatePublished - May 2003
Externally publishedYes

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Mucosal Immunity
Parenteral Nutrition
Enteral Nutrition
Lymphocytes
Lymphoid Tissue
Peyer's Patches
Immunoglobulin A
Diet
Pseudomonas
Immunity
Mucous Membrane
Lymph Nodes
Population
Small Intestine
Intestines
Immunization
Endothelial Cells
Antigens

ASJC Scopus subject areas

  • Surgery

Cite this

Ikeda, S., Kudsk, K. A., Fukatsu, K., Johnson, C. D., Le, T., Reese, S., ... Pruett, T. L. (2003). Enteral Feeding Preserves Mucosal Immunity Despite in Vivo MAdCAM-1 Blockade of Lymphocyte Homing. Annals of Surgery, 237(5), 677-685. https://doi.org/10.1097/00000658-200305000-00010

Enteral Feeding Preserves Mucosal Immunity Despite in Vivo MAdCAM-1 Blockade of Lymphocyte Homing. / Ikeda, Shigeo; Kudsk, Kenneth A.; Fukatsu, Kazuhiko; Johnson, Cheryl D.; Le, Tho; Reese, Shannon; Zarzaur, Ben L.; Garrison, R. Neal; Herndon, David; Watkins, George M.; Pruett, Timothy L.

In: Annals of Surgery, Vol. 237, No. 5, 05.2003, p. 677-685.

Research output: Contribution to journalArticle

Ikeda, S, Kudsk, KA, Fukatsu, K, Johnson, CD, Le, T, Reese, S, Zarzaur, BL, Garrison, RN, Herndon, D, Watkins, GM & Pruett, TL 2003, 'Enteral Feeding Preserves Mucosal Immunity Despite in Vivo MAdCAM-1 Blockade of Lymphocyte Homing', Annals of Surgery, vol. 237, no. 5, pp. 677-685. https://doi.org/10.1097/00000658-200305000-00010
Ikeda, Shigeo ; Kudsk, Kenneth A. ; Fukatsu, Kazuhiko ; Johnson, Cheryl D. ; Le, Tho ; Reese, Shannon ; Zarzaur, Ben L. ; Garrison, R. Neal ; Herndon, David ; Watkins, George M. ; Pruett, Timothy L. / Enteral Feeding Preserves Mucosal Immunity Despite in Vivo MAdCAM-1 Blockade of Lymphocyte Homing. In: Annals of Surgery. 2003 ; Vol. 237, No. 5. pp. 677-685.
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AU - Ikeda, Shigeo

AU - Kudsk, Kenneth A.

AU - Fukatsu, Kazuhiko

AU - Johnson, Cheryl D.

AU - Le, Tho

AU - Reese, Shannon

AU - Zarzaur, Ben L.

AU - Garrison, R. Neal

AU - Herndon, David

AU - Watkins, George M.

AU - Pruett, Timothy L.

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N2 - Objective: To determine the influence of route of nutrition on gut mucosal addressin cellular adhesion molecule-1 (MAdCAM-1) expression and the effect of MAdCAM-1 blockade on gut-associated lymphoid tissue (GALT) lymphocyte populations and established respiratory antibacterial immunity. Summary: Background Data Lymphocytes, sensitized to antigens in Peyer's patches, migrate via mesenteric lymph nodes and home to intestinal lamina propria. MAdCAM-1 located on endothelial cells regulates this trafficking. Experimentally, parenteral nutrition (PN) decreases GALT cell mass and mucosal immunity when compared with enteral feeding. Methods: In experiment 1, MAdCAM-1 expression was quantified in 32 mice after 4 days of feeding chow, a complex diet, intragastric (IG)-PN, or PN. In experiment 2, MAdCAM-1 was measured in 102 mice 0, 4, 8, 12, 24, 48, or 72 hours after starting PN and at 0, 4, 8, 12, 24, or 48 hours after reinstituting chow following 5 days of PN. In experiment 3, 56 mice received chow, PN, chow + MECA-367 (anti-MAdCAM-1 mAb), or chow + Isotype control Ab (IsoAb) for 5 days, followed by Peyer's patches, lamina propria, and intraepithelial lymphocyte yield with respiratory and intestinal IgA levels. In experiment 4, 10 days after Pseudomonas immunization, mice received chow + MECA-367 or chow + IsoAb for 4 days followed by 1.2 × 108 Pseudomonas intratracheally. Results: Diet and route affect MAdCAM-1 expression (chow > complex diet > IG-PN > PN). Decreased MAdCAM-1 expression occurred within hours of starting PN in Peyer's patches, but not mesenteric lymph nodes or the intestine, and recovered quickly with enteral refeeding. MAdCAM-1 blockade reduced all GALT populations. Blockade had little effect on IgA levels and partially impaired the late response of established respiratory immunity. Conclusions: Enteral feeding affects MAdCAM-1 expression. Complete MAdCAM-1 blockade reduces GALT lymphocytes to PN levels, but the chow feeding stimulus preserves IgA and early antibacterial resistance, implying the existence of non-MAdCAM-1 mechanisms to preserve mucosal immunity.

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